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Nanog 抗体 (AA 71-170)

Name: Nanog 抗体 (AA 71-170)
SKU: ABIN728758
Availability: OutOfStock

This anti-Nanog antibody is a 兔多克隆 antibody detecting Nanog in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) 和 IHC (fro). Suitable for 人和小鼠. This Primary Antibody has been cited in 3+ publications.

Lane 1: A549 cell lysates probed with Rabbit Anti-Nanog Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C. (Nanog 抗体 (AA 71-170))

Formalin-fixed and paraffin embedded mouse tooth germ tissue labeled with Anti-Nanog Polyclonal Antibody (ABIN728758), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (Nanog 抗体 (AA 71-170))

IHC

Formalin-fixed and paraffin embedded mouse tooth germ tissue labeled with Anti-Nanog Polyclonal Antibody , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (Nanog 抗体 (AA 71-170))

IHC (p)

HeLa cells probed with Estrogen Receptor alpha + beta Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange) (Nanog 抗体 (AA 71-170))

4 images

(3 references)

产品编号 ABIN728758

发货至: 中国

Datasheet as PDF

Quick Overview for Nanog 抗体 (AA 71-170) (ABIN728758)

抗原

See all Nanog (NANOG) 抗体

Nanog (NANOG) (Nanog Homeobox (NANOG))

适用

人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This Nanog antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Discover our top product NANOG Primary Antibody

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AA 71-170

交叉反应

人, 小鼠

预测反应

Rat,Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse Nanog

亚型

IgG
anti-Nanog Homeobox (NANOG) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, ICC, IHC (p), IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 101-200) antibody
NANOG 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 1-305) antibody
NANOG 适用: 人 WB, ELISA, IF, FACS, ICC 宿主: 小鼠 Monoclonal 8A1D11 unconjugated

anti-Nanog Homeobox (NANOG) (AA 15-49) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated

anti-Nanog Homeobox (NANOG) (Internal Region) antibody
Verified NANOG 适用: 人 WB, ELISA, IF, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 1-100) antibody
NANOG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 20-166) antibody
NANOG 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1E6C4 unconjugated

anti-Nanog Homeobox (NANOG) (AA 267-292) antibody
NANOG 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
El-Badawy, Ghoneim, Gabr, Salah, Mohamed, Amer, El-Badri: "Cancer cell-soluble factors reprogram mesenchymal stromal cells to slow cycling, chemoresistant cells with a more stem-like state." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 254, (2017) (PubMed).

Liu, Zhang, Wang, Yang, Tan: "JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells." in: Acta pharmacologica Sinica, Vol. 36, Issue 9, pp. 1099-106, (2016) (PubMed).

Liu, Li, Liu, Sui, Liu, Xiao, Zheng, Chen, Yao, Xing, Zhou, Li: "A signature for induced pluripotent stem cell-associated genes in colorectal cancer." in: Medical oncology (Northwood, London, England), Vol. 30, Issue 1, pp. 426, (2013) (PubMed).
抗原

Nanog (NANOG) (Nanog Homeobox (NANOG))

别名

Nanog

背景

Synonyms: ENK, ecat4, 2412E2Rik, Homeobox protein NANOG, ES cell-associated protein 4, Early embryo specific expression NK-type homeobox protein, Homeobox transcription factor Nanog, Nanog
Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

基因ID

71950

UniProt

Q80Z64

途径

Stem Cell Maintenance