EEF2 抗体 (N-Term)
Quick Overview for EEF2 抗体 (N-Term) (ABIN7603141)
抗原
See all EEF2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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原理
- Anti-EEF2 Picoband® Antibody (monoclonal, 5F5)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-EEF2 Picoband® Antibody (monoclonal, 5F5) (ABIN7603141). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human EEF2/Elongation factor 2, identical to the related mouse and rat sequences.
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亚型
- IgG1
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anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 32-233) antibody
EEF2 适用: 人 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) antibodyEEF2 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) antibodyEEF2 适用: 人 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 5B6 unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) antibodyEEF2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 1-290) antibodyEEF2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 2-858) antibodyEEF2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 36-85) antibodyEEF2 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 小鸡, 猴, 犬, Pig, 非洲爪蟾, 豚鼠, 马, Drosophila melanogaster, Bat, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (Ser311) antibodyEEF2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (N-Term) antibodyEEF2 适用: 人, 小鼠, 大鼠, Drosophila melanogaster, C. elegans WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (N-Term), (pSer56) antibodyEEF2 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 小鸡, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Huma
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse, Rat
1. Davydova, E., Ho, A. Y. Y., Malecki, J., Moen, A., Enserink, J. M., Jakobsson, M. E., Loenarz, C., Falnes, P. O. Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2). J. Biol. Chem. 289: 30499-30510, 2014. 2. Hekman, K. E., Yu, G.-Y., Brown, C. D., Zhu, H., Du, X., Gervin, K., Undlien, D. E., Peterson, A., Stevanin, G., Clark, H. B., Pulst, S. M., Bird, T. D., White, K. P., Gomez, C. M. A conserved eEF2 coding variant in SCA26 leads to loss of translational fidelity and increased susceptibility to proteostatic insult. Hum. Molec. Genet. 21: 5472-5483, 2012. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))
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别名
- EEF2
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背景
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Synonyms: Dihydrofolate reductase, DHFR
Tissue Specificity: Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.
Background: Eukaryotic elongation factor 2is aproteinthat in humans is encoded by theEEF2gene. This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
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分子量
- 95 kDa
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基因ID
- 1938
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UniProt
- P13639
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途径
- AMPK Signaling
抗原
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