ATG7 抗体 (N-Term)
Quick Overview for ATG7 抗体 (N-Term) (ABIN7603109)
抗原
See all ATG7 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- Anti-ATG7 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-ATG7 Antibody Picoband® (ABIN7603109). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human ATG7, identical to the related mouse and rat sequences.
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亚型
- IgG
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anti-ATG7 Autophagy Related 7 (ATG7) (AA 500-676) antibody
ATG7 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG7 Autophagy Related 7 (ATG7) (AA 477-698) antibodyATG7 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-ATG7 Autophagy Related 7 (ATG7) (AA 540-569), (C-Term) antibodyATG7 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7474 unconjugated
anti-ATG7 Autophagy Related 7 (ATG7) (AA 22-51) antibodyATG7 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ATG7 Autophagy Related 7 (ATG7) (AA 494-523) antibodyATG7 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG7 Autophagy Related 7 (ATG7) antibodyATG7 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-ATG7 Autophagy Related 7 (ATG7) (AA 558-703) antibodyATG7 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 7D3E5 unconjugated
anti-ATG7 Autophagy Related 7 (ATG7) (N-Term) antibodyATG7 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ATG7 Autophagy Related 7 (ATG7) (N-Term) antibody (HRP)ATG7 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP
anti-ATG7 Autophagy Related 7 (ATG7) (N-Term) antibody (FITC)ATG7 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal FITC
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse, Rat
1. Komatsu, M., Wang, Q. J., Holstein, G. R., Friedrich, V. L., Jr., Iwata, J., Kominami, E., Chait, B. T., Tanaka, K., Yue, Z. Essential role for autophagy protein Atg7 in the maintenance of axonal homeostasis and the prevention of axonal degeneration. Proc. Nat. Acad. Sci. 104: 14489-14494, 2007. 2. Lee, I. H., Kawai, Y., Fergusson, M. M., Rovira, I. I., Bishop, A. J. R., Motoyama, N., Cao, L., Finkel, T. Atg7 modulates p53 activity to regulate cell cycle and survival during metabolic stress. Science 336: 225-228, 2012. Note: Erratum: Science 337: 910 only, 2012. Erratum: Science 341: 457 only, 2013. 3. Nishida, Y., Arakawa, S., Fujitani, K., Yamaguchi, H., Mizuta, T., Kanaseki, T., Komatsu, M., Otsu, K., Tsujimoto, Y., Shimizu, S. Discovery of Atg5/Atg7-independent alternative macroautophagy. Nature 461: 654-658, 2009. Note: Erratum: Nature 533: 130 only, 2016. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ATG7 (ATG7 Autophagy Related 7 (ATG7))
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别名
- ATG7
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背景
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Synonyms: Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, APG7-like, hAGP7, Ubiquitin-activating enzyme E1-like protein, ATG7, APG7L
Tissue Specificity: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Background: Autophagy related 7 is a protein in humans encoded by ATG7 gene. It is mapped to 3p25.3. This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.
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分子量
- 78 kDa
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基因ID
- 10533
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UniProt
- O95352
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途径
- Response to Water Deprivation, Autophagy
抗原
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