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Cytokeratin 5 抗体 (Middle Region)

This anti-Cytokeratin 5 antibody is a 小鼠 单克隆 antibody detecting Cytokeratin 5 in WB, IHC, IF, FACS 和 ICC. Suitable for 人.
产品编号 ABIN7602993
发货至: 中国

Quick Overview for Cytokeratin 5 抗体 (Middle Region) (ABIN7602993)

抗原

See all Cytokeratin 5 (KRT5) 抗体
Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

适用

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宿主

  • 108
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小鼠

克隆类型

  • 93
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单克隆

标记

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This Cytokeratin 5 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

克隆位点

9H2F8
  • 抗原表位

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    Middle Region

    原理

    Anti-Cytokeratin 5 Antibody Picoband® (monoclonal, 9H2F8)

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-Cytokeratin 5 Antibody Picoband® (monoclonal, 9H2F8) (ABIN7602993). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence in the middle region of human Cytokeratin 5, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

    亚型

    IgG2a
  • 应用备注

    Western blot, 0.25-0.5 μg/mL, Human
    Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x6 cells, Human
    1. "Entrez Gene: KRT5 keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)". 2. Eckert RL, Rorke EA (Jun 1988). "The sequence of the human epidermal 58-kD (#5) type II keratin reveals an absence of 5' upstream sequence conservation between coexpressed epidermal keratins". Dna. 7 (5): 337-45. 3. Lersch R, Fuchs E (Jan 1988). "Sequence and expression of a type II keratin, K5, in human epidermal cells". Molecular and Cellular Biology. 8 (1): 486-93.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • 抗原

    Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

    别名

    KRT5

    背景

    Synonyms: Interferon gamma, IFN-gamma, IFNG

    Tissue Specificity: Released primarily from activated T lymphocytes.

    Background: Cytokeratin 5, also known as KRT5, K5, or CK5, is a protein that is encoded in humans by the KRT5 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

    分子量

    62 kDa

    基因ID

    3852

    UniProt

    P13647
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