ZEB1 抗体 (Middle Region)
Quick Overview for ZEB1 抗体 (Middle Region) (ABIN7602984)
抗原
See all ZEB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- Anti-AREB6/ZEB1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-AREB6/ZEB1 Antibody Picoband® (ABIN7602984). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human ZEB1, which shares 94.9% and 100% amino acid (aa) sequence identity with rat ZEB1.
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亚型
- IgG
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anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (AA 967-1108) antibody
ZEB1 适用: 人 IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2A8A6 unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (AA 801-900) antibodyZEB1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4C4 unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (N-Term) antibodyZEB1 适用: 人, 小鼠, 大鼠, 兔, 犬, Cow, 豚鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (AA 1-260) antibodyZEB1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) antibodyKO Validated ZEB1 适用: 人 WB, IF, IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) antibodyZEB1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (Internal Region) antibodyZEB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (N-Term) antibodyZEB1 适用: 人, 小鼠, 大鼠, 兔, 犬, Cow, Pig, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (Internal Region) antibodyVerified ZEB1 适用: 人, 小鼠 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (N-Term) antibodyZEB1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Krafchak, C. M., Pawar, H., Moroi, S. E., Sugar, A., Lichter, P. R., Mackey, D. A., Mian, S., Nairus, T., Elner, V., Schteingart, M. T., Downs, C. A., Kijek, T. G., and 9 others. Mutations in TCF8 cause posterior polymorphous corneal dystrophy and ectopic expression of COL4A3 by corneal endothelial cells. Am. J. Hum. Genet. 77: 694-708, 2005. 2. Nishimura, G., Manabe, I., Tsushima, K., Fujiu, K., Oishi, Y., Imai, Y., Maemura, K., Miyagishi, M., Higashi, Y., Kondoh, H., Nagai, R. Delta-EF1 mediates TGF-beta signaling in vascular smooth muscle cell differentiation. Dev. Cell 11: 93-104, 2006. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))
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别名
- ZEB1
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背景
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Synonyms: Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8, ZEB1, AREB6, TCF8
Tissue Specificity: Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
Background: ZEB1 (Zinc Finger E Box-Binding Homeobox 1), also called TCF8, NIL2A or DELTA-EF1, is a protein that in humans is encoded by the ZEB1 gene. Fluorescence in situ hybridization localized the ZEB1 gene to chromosome 10p11.2. Krafchak et al. (2005) demonstrated a complex (core plus secondary) binding site for TCF8 in the promoter of the COL4A3 gene, mutant in Alport syndrome and which encodes collagen type IV alpha-3. They detected expression of TCF8 in cornea. Nishimura et al. (2006) found that delta-Ef1 was upregulated during differentiation in a mouse smooth muscle cell (SMC) line.
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分子量
- 200 kDa
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基因ID
- 6935
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UniProt
- P37275
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途径
- Regulation of Muscle Cell Differentiation
抗原
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