ZEB1 抗体 (Middle Region)
Quick Overview for ZEB1 抗体 (Middle Region) (ABIN7602984)
抗原
See all ZEB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- Anti-AREB6/ZEB1 Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-AREB6/ZEB1 Antibody (ABIN7602984). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human ZEB1, which shares 94.9% and 100% amino acid (aa) sequence identity with rat ZEB1.
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亚型
- IgG
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anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (AA 967-1108) antibody
ZEB1 适用: 人 IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2A8A6 unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (AA 801-900) antibodyZEB1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4C4 unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (N-Term) antibodyZEB1 适用: 人, 小鼠, 大鼠, 兔, 犬, Cow, 豚鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (AA 1-260) antibodyZEB1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) antibodyKO Validated ZEB1 适用: 人 WB, IF, ICC, IP, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (Internal Region) antibodyZEB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) antibodyZEB1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (N-Term) antibodyZEB1 适用: 人, 小鼠, 大鼠, 兔, 犬, Cow, Pig, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (Internal Region) antibodyVerified ZEB1 适用: 人, 小鼠 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (N-Term) antibodyZEB1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))
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别名
- ZEB1
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背景
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Synonyms: Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8, ZEB1, AREB6, TCF8
Tissue Specificity: Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
Background: ZEB1 (Zinc Finger E Box-Binding Homeobox 1), also called TCF8, NIL2A or DELTA-EF1, is a protein that in humans is encoded by the ZEB1 gene. Fluorescence in situ hybridization localized the ZEB1 gene to chromosome 10p11.2. Krafchak et al. (2005) demonstrated a complex (core plus secondary) binding site for TCF8 in the promoter of the COL4A3 gene, mutant in Alport syndrome and which encodes collagen type IV alpha-3. They detected expression of TCF8 in cornea. Nishimura et al. (2006) found that delta-Ef1 was upregulated during differentiation in a mouse smooth muscle cell (SMC) line.
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分子量
- 200 kDa
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基因ID
- 6935
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UniProt
- P37275
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途径
- Regulation of Muscle Cell Differentiation
抗原
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