T-Bet 抗体 (Middle Region)
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北京 101111
Quick Overview for T-Bet 抗体 (Middle Region) (ABIN7602980)
抗原
See all T-Bet 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- Anti-T-bet/Tbx21 Antibody
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-T-bet/Tbx21 Antibody (ABIN7602980). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human T-bet/Tbx21, identical to the related mouse and rat sequences.
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse, Rat
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- T-Bet
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别名
- TBX21
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背景
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Synonyms: T-box transcription factor TBX21, T-box protein 21, T-cell-specific T-box transcription factor T-bet, Transcription factor TBLYM, TBX21, TBET, TBLYM
Tissue Specificity: Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3.
Background: T-box transcription factor TBX21 is a protein that in humans is encoded by the TBX21 gene. It is mapped to 17q21.32. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.
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分子量
- 65 kDa
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基因ID
- 30009
抗原
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