ATG4A 抗体 (C-Term)
Quick Overview for ATG4A 抗体 (C-Term) (ABIN7602947)
抗原
See all ATG4A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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原理
- Anti-Atg4A Monoclonal Antibody
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产品特性
- Anti-Atg4A Monoclonal Antibody (ABIN7602947). Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human Atg4A The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.
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亚型
- IgG
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应用备注
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WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50 -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
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浓度
- Lot specific
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
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- ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))
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别名
- ATG4A
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背景
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Synonyms: Death domain-containing protein CRADD,Caspase and RIP adapter with death domain,RIP-associated protein with a death domain,CRADD,RAIDD,
Tissue Specificity: Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
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分子量
- 45 kDa
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途径
- Autophagy
抗原
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