GAPDH 抗体 (C-Term) (HRP)
Quick Overview for GAPDH 抗体 (C-Term) (HRP) (ABIN7602946)
抗原
See all GAPDH 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Anti-GAPDH Antibody Picoband® (HRP)
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交叉反应
- 小鸡, 人, 猴, 小鼠, 大鼠, 斑马鱼
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交叉反应 (详细)
- HRP-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.
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产品特性
- Anti-GAPDH Antibody (HRP) (-HRP). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- HRP-GAPDH antibody is affinity chromatography purified via peptide column.
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免疫原
- HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.
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亚型
- IgG
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应用备注
- Western blot, 0.02-0.1 μg/mL
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 0.5 mg/mL
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缓冲液
- Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- HRP-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
- GAPDH
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背景
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Synonyms: HRP-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
Tissue Specificity: Widely expressed, with the highest expression in lung, spleen and thymus.
Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Sequence Similarities: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
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分子量
- 36 kDa
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UniProt
- P04406
抗原
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