GPBAR1 抗体 (C-Term)
Quick Overview for GPBAR1 抗体 (C-Term) (ABIN7602825)
抗原
See all GPBAR1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Anti-GPCR TGR5/GPBAR1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-GPCR TGR5/GPBAR1 Antibody Picoband® (ABIN7602825). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human GPCR TGR5/GPBAR1, which shares 100% and 92.9% amino acid (aa) sequence identity with human and rat GPCR TGR5/GPBAR1, respectively.
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亚型
- IgG
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anti-G Protein-Coupled Bile Acid Receptor 1 (GPBAR1) (C-Term) antibody
GPBAR1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Bile Acid Receptor 1 (GPBAR1) (Cytoplasmic Domain) antibodyGPBAR1 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Mouse, Rat
1. Maruyama, T., Miyamoto, Y., Nakamura, T., Tamai, Y., Okada, H., Sugiyama, E., Nakamura, T., Itadani, H., Tanaka, K. Identification of membrane-type receptor for bile acids (M-BAR). Biochem. Biophys. Res. Commun. 298: 714-719, 2002. 2. Watanabe, M., Houten, S. M., Mataki, C., Christoffolete, M. A., Kim, B. W., Sato, H., Messaddeq, N., Harney, J. W., Ezaki, O., Kodama, T., Schoonjans, K., Bianco, A. C., Auwerx, J. Bile acids induce energy expenditure by promoting intracellular thyroid hormone activation. Nature 439: 484-489, 2006. 3. Yoneno, K., Hisamatsu, T., Shimamura, K., Kamada, N., Ichikawa, R., Kitazume, M. T., Mori, M., Uo, M., Namikawa, Y., Matsuoka, K., Sato, T., Koganei, K., Sugita, A., Kanai, T., Hibi, T. TGR5 signalling inhibits the production of pro-inflammatory cytokines by in vitro differentiated inflammatory and intestinal macrophages in Crohn's disease. Immunology 139: 19-29, 2013. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- GPBAR1 (G Protein-Coupled Bile Acid Receptor 1 (GPBAR1))
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别名
- GPBAR1
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背景
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Synonyms: Insulin-like growth factor-binding protein 5,IBP-5,IGF-binding protein 5,IGFBP-5,IGFBP5,IBP5,
Tissue Specificity: Osteosarcoma, and at lower levels in liver, kidney and brain.
Background: The G protein-coupled bile acid receptor 1 (GPBAR1) also known G-protein coupled receptor 19 (GPCR19), membrane-type receptor for bile acids (M-BAR) or TGR5 as is a protein that in humans is encoded by the GPBAR1 gene. This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein.
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分子量
- 46-48 kDa
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基因ID
- 227289
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途径
- WNT signaling, Hormone Transport, Sensory Perception of Sound
抗原
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