UCHL1 抗体 (C-Term)
Our Local Distributor
北京 101111
Quick Overview for UCHL1 抗体 (C-Term) (ABIN7602794)
抗原
See all UCHL1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- C-Term
-
原理
- Anti-PGP9.5 Antibody (monoclonal, 3E4)
-
交叉反应 (详细)
- No cross-reactivity with other proteins.
-
产品特性
- Anti-PGP9.5 Antibody (monoclonal, 3E4) (ABIN7602794). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human PGP9.5, different from the related mouse and rat sequences by two amino acids.
-
亚型
- IgG1
-
-
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
-
别名
- UCHL1
-
背景
-
Synonyms: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial, CLPX
Tissue Specificity: Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.
Background: UCH-L1, also known as PGP9.5, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is abundantly present in all neurons (accounts for 1-2 % of total brain protein), expressed specifically in neurons and testis/ovary. The catalytic triad of UCH-L1 contains a cysteine at position 90, an aspartate at position 176, and a histidine at position 161 that are responsible for its hydrolase activity.
-
分子量
- 27 kDa
-
基因ID
- 7345
-
UniProt
- P09936
-
途径
- Feeding Behaviour
抗原
-