ULK1 抗体 (C-Term)
Quick Overview for ULK1 抗体 (C-Term) (ABIN7602755)
抗原
See all ULK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Anti-ULK1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-ULK1 Antibody Picoband® (ABIN7602755). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human ULK1, which shares 81.8% amino acid (aa) sequence identity with both mouse and rat ULK1.
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亚型
- IgG
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anti-Unc-51 Like Kinase 1 (ULK1) (AA 951-1050) antibody
ULK1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Unc-51 Like Kinase 1 (ULK1) (AA 602-715) antibodyULK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H8 unconjugated
anti-Unc-51 Like Kinase 1 (ULK1) (AA 400-499) antibodyULK1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Unc-51 Like Kinase 1 (ULK1) antibodyULK1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Unc-51 Like Kinase 1 (ULK1) (pSer556) antibodyULK1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Unc-51 Like Kinase 1 (ULK1) (AA 567-577) antibodyULK1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Unc-51 Like Kinase 1 (ULK1) (AA 567-577) antibody (HRP)ULK1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal HRP
anti-Unc-51 Like Kinase 1 (ULK1) (AA 567-577) antibody (FITC)ULK1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal FITC
anti-Unc-51 Like Kinase 1 (ULK1) (AA 567-577) antibody (Biotin)ULK1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Biotin
anti-Unc-51 Like Kinase 1 (ULK1) (AA 567-577) antibody (PE)ULK1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal PE
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Egan, D. F., Shackelford, D. B., Mihaylova, M. M., Gelino, S., Kohnz, R. A., Mair, W., Vasquez, D. S., Joshi, A., Gwinn, D. M., Taylor, R., Asara, J. M., Fitzpatrick, J., Dillin, A., Viollet, B., Kundu, M., Hansen, M., Shaw, R. J. Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase connects energy sensing to mitophagy. Science 331: 456-461, 2011. 2. Horne, D. J., Graustein, A. D., Shah, J. A., Peterson, G., Savlov, M., Steele, S., Narita, M., Hawn, T. R. Human ULK1 variation and susceptibility to Mycobacterium tuberculosis infection. J. Infect. Dis. 214: 1260-1267, 2016. 3. Kundu, M., Lindsten, T., Yang, C.-Y., Wu, J., Zhao, F., Zhang, J., Selak, M. A., Ney, P. A., Thompson, C. B. Ulk1 plays a critical role in the autophagic clearance of mitochondria and ribosomes during reticulocyte maturation. Blood 112: 1493-1502, 2008. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ULK1 (Unc-51 Like Kinase 1 (ULK1))
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别名
- ULK1
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背景
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Synonyms: Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, ATG1, hATG1, Unc-51-like kinase 1, ULK1, KIAA0722
Tissue Specificity: Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
Background: ULK1 is an enzyme that in humans is encoded by the ULK1 gene. It is mapped to 12q24.33. Unc-51 like autophagy activating kinase (ULK1/2) are two similar isoforms of an enzyme that in humans are encoded by the ULK1/2 genes. It is specifically a kinase that is involved with autophagy, particularly in response to amino acid withdrawal. Not many studies have been done comparing the two isoforms, but some differences have been recorded.
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分子量
- 120 kDa
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基因ID
- 8408
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UniProt
- O75385
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途径
- Regulation of Cell Size, Autophagy
抗原
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