HMGB1 抗体 (C-Term)
Quick Overview for HMGB1 抗体 (C-Term) (ABIN7602748)
抗原
See all HMGB1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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原理
- Anti-HMGB1 Antibody Picoband® (monoclonal, 5H3)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-HMGB1 Antibody Picoband® (monoclonal, 5H3) (ABIN7602748). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human HMGB1, identical to the related mouse and rat sequences.
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亚型
- IgG2b
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anti-High Mobility Group Box 1 (HMGB1) (AA 75-170) antibody
HMGB1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) antibodyKO Validated HMGB1 适用: 人 WB, IHC, IF, ICC, IHC (p), IHC (wm), IHC (ff) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-90) antibodyHMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 9-163) antibodyHMGB1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E6-E10 unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1D5 unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 124-154), (C-Term) antibodyHMGB1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Ferrari S, Finelli P, Rocchi M, Bianchi ME (July 1996). "The active gene that encodes human high mobility group 1 protein (HMG1) contains introns and maps to chromosome 13". Genomics. 35 (2): 367-71. 2. Klune JR, Dhupar R, Cardinal J, Billiar TR, Tsung A (2008)."HMGB1: endogenous danger signaling". Mol. Med. 14 (7-8): 476-84. 3. Yang H, Hreggvidsdottir HS, Palmblad K, Wang H, Ochani M, Li J, Lu B, Chavan S, Rosas-Ballina M, Al-Abed Y, Akira S, Bierhaus A, Erlandsson-Harris H, Andersson U, Tracey KJ (June 2010). "A critical cysteine is required for HMGB1 binding to Toll-like receptor 4 and activation of macrophage cytokine release". Proc. Natl. Acad. Sci. U.S.A. 107 (26): 11942-7. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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别名
- HMGB1
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背景
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Synonyms: High mobility group protein B1, High mobility group protein 1, HMG-1, HMGB1, HMG1
Tissue Specificity: Ubiquituous. Expressed in platelets.
Background: High mobility group box 1 protein, also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a protein that in humans is encoded by the HMGB1 gene. This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.
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分子量
- 25 kDa
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基因ID
- 3146
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UniProt
- P09429
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途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
抗原
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