LIMA1 抗体 (AA 9-744)
Quick Overview for LIMA1 抗体 (AA 9-744) (ABIN7602639)
抗原
See all LIMA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
 - AA 9-744
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原理
 - Anti-EPLIN/LIMA1 Antibody Picoband®
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交叉反应 (详细)
 - No cross-reactivity with other proteins.
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产品特性
 - Anti-EPLIN/LIMA1 Antibody Picoband® (ABIN7602639). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
 - Immunogen affinity purified.
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免疫原
 - E.coli-derived human EPLIN/LIMA1 recombinant protein (Position: R9-E744).
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亚型
 - IgG
 
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应用备注
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                        Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, S., Maul, R. S., Kim, H. R., Chang, D. D. Characterization of the human EPLIN (Epithelial Protein Lost in Neoplasm) gene reveals distinct promoters for the two EPLIN isoforms. Gene 248: 69-76, 2000. 2. Maul, R. S., Chang, D. D. EPLIN, epithelial protein lost in neoplasm. Oncogene 18: 7838-7841, 1999. 3. Maul, R. S., Song, Y., Amann, K. J., Gerbin, S. C., Pollard, T. D., Chang, D. D. EPLIN regulates actin dynamics by cross-linking and stabilizing filaments. J. Cell Biol. 160: 399-407, 2003. - 
                                            
限制
 - 仅限研究用
 
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状态
 - Lyophilized
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溶解方式
 - Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
 - 500 μg/mL
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缓冲液
 - Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
 - 4 °C,-20 °C
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储存方法
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                        At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. 
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. 
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- LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))
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别名
 - LIMA1
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背景
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Synonyms: Zinc finger protein Helios, Ikaros family zinc finger protein 2, IKZF2, HELIOS, ZNFN1A2
Tissue Specificity: Ubiquitous. High expression in liver, spleen, pancreas and kidney.
Background: LIM domain and actin-binding protein 1 is a protein that in humans is encoded by the LIMA1 gene. This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated.
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分子量
 - 85 kDa, 100 kDa
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基因ID
 - 51474
 
抗原
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