NDC80 抗体 (AA 87-602)
Quick Overview for NDC80 抗体 (AA 87-602) (ABIN7602585)
抗原
See all NDC80 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 87-602
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原理
- Anti-HEC1/HEC/NDC80 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-HEC1/HEC/NDC80 Antibody Picoband® (ABIN7602585). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human HEC1/HEC/NDC80 recombinant protein (Position: N87-E602).
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亚型
- IgG
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anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (AA 545-642) antibody
NDC80 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A10 unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (C-Term) antibodyNDC80 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (C-Term) antibodyVerified NDC80 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) antibodyNDC80 适用: 人 WB, ICC, DB 宿主: 小鼠 Monoclonal 2497C3a unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (AA 508-539) antibodyNDC80 适用: 人 WB 宿主: 兔 Polyclonal RB55345 unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (AA 594-628) antibodyNDC80 适用: 人 WB 宿主: 兔 Polyclonal RB53951 unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (AA 1-642) antibodyNDC80 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (AA 1-642) antibodyNDC80 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (AA 343-642) antibodyNDC80 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) antibodyNDC80 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen Y, Riley DJ, Chen PL, Lee WH (October 1997). "HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis". Molecular and Cellular Biology. 17 (10): 6049-56. 2. Martin-Lluesma S, Stucke VM, Nigg EA (September 2002). "Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2". Science. 297 (5590): 2267-70. 3. Chen Y, Sharp ZD, Lee WH (September 1997). "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins". The Journal of Biological Chemistry. 272 (38): 24081-7. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))
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别名
- NDC80
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背景
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Synonyms: Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, NDC80, HEC, HEC1, KNTC2
Tissue Specificity: Expression peaks in mitosis.
Background: Kinetochore protein NDC80 homolog is a protein that in humans is encoded by the NDC80 gene. It is mapped to 18p11.32. This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.
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分子量
- 74 kDa
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基因ID
- 10403
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UniProt
- O14777
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途径
- Maintenance of Protein Location
抗原
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