EIF5A 抗体 (AA 86-154)
Quick Overview for EIF5A 抗体 (AA 86-154) (ABIN7602568)
抗原
See all EIF5A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 86-154
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原理
- Anti-EIF5A Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-EIF5A Antibody Picoband® (ABIN7602568). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human EIF5A recombinant protein (Position: R86-K154).
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 5A (EIF5A) (AA 1-154) antibody
EIF5A 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 5A (EIF5A) (AA 1-154) antibodyEIF5A 适用: 人 ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 4E10G8 unconjugated
anti-Eukaryotic Translation Initiation Factor 5A (EIF5A) antibodyEIF5A 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Monoclonal 5E1 unconjugated Recombinant Antibody
anti-Eukaryotic Translation Initiation Factor 5A (EIF5A) (AA 1-154) antibodyEIF5A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 8C1 unconjugated
anti-Eukaryotic Translation Initiation Factor 5A (EIF5A) (Internal Region) antibodyVerified EIF5A 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 5A (EIF5A) antibodyEIF5A 适用: 人, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 5A (EIF5A) (AA 2-154) antibodyEIF5A 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 5A (EIF5A) (Internal Region) antibodyEIF5A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 5A (EIF5A) (AA 1-154) antibody (PE)EIF5A 适用: 人 IHC, IF, FACS, ICC 宿主: 兔 Polyclonal PE
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Doerfel, L. K., Wohlgemuth, I., Kothe, C., Peske, F., Urlaub, H., Rodnina, M. V. EF-P is essential for rapid synthesis of proteins containing consecutive proline residues. Science 339: 85-88, 2013. 2. Faundes, V., Jennings, M. D., Crilly, S., Legraie, S., Withers, S. E., Cuvertino, S., Davies, S. J., Douglas, A. G. L., Fry, A. E., Harrison, V., Amiel, J., Lehalle, D., and 12 others. Impaired eIF5A function causes a Mendelian disorder that is partially rescued in model systems by spermidine. Nature Commun. 12: 833, 2021. 3. Gutierrez, E., Shin, B.-S., Woolstenhulme, C. J., Kim, J.-R., Saini, P., Buskirk, A. R., Dever, T. E. eIF5A promotes translation of polyproline motifs. Molec. Cell 51: 35-45, 2013. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))
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别名
- EIF5A
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背景
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Synonyms: Dual specificity mitogen-activated protein kinase kinase 4, MAP kinase kinase 4, MAPKK 4, JNK-activating kinase 1, MAPK/ERK kinase 4, MEK 4, SAPK/ERK kinase 1, SEK1, Stress-activated protein kinase kinase 1, SAPK kinase 1, SAPKK-1, SAPKK1, c-Jun N-terminal kinase kinase 1, JNKK, MAP2K4, JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1
Tissue Specificity: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Background: Eukaryotic translation initiation factor 5A-1 is a protein that in humans is encoded by the EIF5A gene. Eukaryotic initiation factor 5A (eIF5A) is an mRNA-binding protein that is involved in translation elongation and plays an important role in promoting translation of polyproline motifs. The eIF5A (eIF5A1) and eIF5A2 genes encode the two vertebrate eIF5A isoforms. While eIF5A1 is expressed constitutively in all tissues, eIF5A2 is mainly expressed in gonads. eIF5A and eIF5A2 are the only identified proteins that contain the distinctive amino acid hypusine, which is generated posttranslationally from lysine through a highly conserved polyamine metabolism pathway. eIF5A function and hypusine modification are both essential for cell proliferation, as knock down of eIF5A expression or blocking eIF5A hypusine modification suppresses cancer cell proliferation. Interestingly, eIF5A is an identified component of a tumor suppressor network of the polyamine-hypusine axis. Co-suppression of both eIF5A and adenosylmethionine decarboxylase 1 (AMD1) promotes lymphomagenesis in mice, while heterozygous deletions of the corresponding AMD1 and eIF5A genes often occur together in human lymphomas.
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分子量
- 18 kDa
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基因ID
- 1984
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UniProt
- P63241
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途径
- Regulation of Muscle Cell Differentiation
抗原
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