ECSIT 抗体 (AA 83-339)
Quick Overview for ECSIT 抗体 (AA 83-339) (ABIN7602543)
抗原
See all ECSIT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 83-339
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原理
- Anti-ECSIT Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-ECSIT Antibody (ABIN7602543). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human ECSIT recombinant protein (Position: E83-S339).
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亚型
- IgG
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anti-ECSIT (ECSIT) (AA 185-431) antibody
ECSIT 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ECSIT (ECSIT) (AA 51-100) antibodyECSIT 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-ECSIT (ECSIT) (AA 20-431) antibodyECSIT 适用: 人 ELISA, PLA 宿主: 小鼠 Monoclonal 1B8 unconjugated
anti-ECSIT (ECSIT) (AA 241-290) antibodyECSIT 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-ECSIT (ECSIT) (Middle Region) antibodyECSIT 适用: 人, 小鼠, 大鼠, Cow, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-ECSIT (ECSIT) (C-Term) antibodyECSIT 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-ECSIT (ECSIT) antibodyECSIT 适用: 人 WB, IF/ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- ECSIT
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别名
- ECSIT
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背景
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Synonyms: DAZ-associated protein 1, Deleted in azoospermia-associated protein 1, DAZAP1
Tissue Specificity: Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Background: Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial (ECSIT), also known as SITPEC, is a protein that in humans is encoded by the ECSIT gene. Activation of NF-kB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)-6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.
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分子量
- 49 kDa
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基因ID
- 51295
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途径
- TLR signaling, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome
抗原
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