ECSIT 抗体 (AA 83-339)
Quick Overview for ECSIT 抗体 (AA 83-339) (ABIN7602543)
抗原
See all ECSIT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 83-339
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原理
- Anti-ECSIT Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-ECSIT Antibody Picoband® (ABIN7602543). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human ECSIT recombinant protein (Position: E83-S339).
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亚型
- IgG
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anti-ECSIT (ECSIT) (AA 185-431) antibody
ECSIT 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ECSIT (ECSIT) (AA 241-290) antibodyECSIT 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-ECSIT (ECSIT) (AA 20-431) antibodyECSIT 适用: 人 ELISA, PLA 宿主: 小鼠 Monoclonal 1B8 unconjugated
anti-ECSIT (ECSIT) (AA 51-100) antibodyECSIT 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-ECSIT (ECSIT) (AA 50-296) antibodyECSIT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-ECSIT (ECSIT) (Middle Region) antibodyECSIT 适用: 人, 小鼠, 大鼠, Cow, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-ECSIT (ECSIT) (C-Term) antibodyECSIT 适用: 人, 小鼠 WB, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-ECSIT (ECSIT) antibodyECSIT 适用: 人 WB, IF/ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kopp, E., Medzhitov, R., Carothers, J., Xiao, C., Douglas, I., Janeway, C. A., Ghosh, S. ECSIT is an evolutionarily conserved intermediate in the Toll/IL-1 signal transduction pathway. Genes Dev. 13: 2059-2071, 1999. 2. Vogel, R. O., Janssen, R. J. R. J., van den Brand, M. A. M., Dieteren, C. E. J., Verkaart, S., Koopman, W. J. H., Willems, P. H. G. M., Pluk, W., van den Heuvel, L. P. W. J., Smeitink, J. A. M., Nijtmans, L. G. J. Cytosolic signaling protein Ecsit also localizes to mitochondria where it interacts with chaperone NDUFAF1 and functions in complex I assembly. Genes Dev. 21: 615-624, 2007. 3. West, A. P., Brodsky, I. E., Rahner, C., Woo, D. K., Erdjument-Bromage, H., Tempst, P., Walsh, M. C., Choi, Y., Shadel, G. S., Ghosh, S. TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. Nature 472: 476-480, 2011. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- ECSIT
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别名
- ECSIT
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背景
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Synonyms: DAZ-associated protein 1, Deleted in azoospermia-associated protein 1, DAZAP1
Tissue Specificity: Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Background: Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial (ECSIT), also known as SITPEC, is a protein that in humans is encoded by the ECSIT gene. Activation of NF-kB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)-6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.
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分子量
- 49 kDa
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基因ID
- 51295
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途径
- TLR signaling, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome
抗原
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