Ancient Ubiquitous Protein 1 抗体 (AA 77-399)
Quick Overview for Ancient Ubiquitous Protein 1 抗体 (AA 77-399) (ABIN7602440)
抗原
See all Ancient Ubiquitous Protein 1 (Aup1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 77-399
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原理
- Anti-AUP1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-AUP1 Antibody Picoband® (ABIN7602440). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human AUP1 recombinant protein (Position: A77-R399).
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亚型
- IgG
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anti-Ancient Ubiquitous Protein 1 (Aup1) (AA 229-410) antibody
Aup1 适用: 人, 小鼠 WB, ELISA 宿主: 小鼠 Monoclonal 1H6A1 unconjugated
anti-Ancient Ubiquitous Protein 1 (Aup1) antibodyAup1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ancient Ubiquitous Protein 1 (Aup1) (AA 201-410) antibodyAup1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ancient Ubiquitous Protein 1 (Aup1) antibodyAup1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ancient Ubiquitous Protein 1 (Aup1) (AA 201-410) antibodyAup1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Jang, W., Weber, J. S., Bashir, R., Bushby, K., Meisler, M. H. Aup1, a novel gene on mouse chromosome 6 and human chromosome 2p13. Genomics 36: 366-368, 1996. 2. Mueller, B., Klemm, E. J., Spooner, E., Claessen, J. H., Ploegh, H. L. SEL1L nucleates a protein complex required for dislocation of misfolded glycoproteins. Proc. Nat. Acad. Sci. 105: 12325-12330, 2008. 3. Spandl, J., Lohmann, D., Kuerschner, L., Moessinger, C., Thiele, C. Ancient ubiquitous protein 1 (AUP1) localizes to lipid droplets and binds the E2 ubiquitin conjugase G2 (Ube2g2) via its G2 binding region. J. Biol. Chem. 286: 5599-5606, 2011. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Ancient Ubiquitous Protein 1 (Aup1)
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别名
- AUP1
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背景
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Synonyms: DnaJ homolog subfamily A member 2, Cell cycle progression restoration gene 3 protein, Dnj3, Dj3, HIRA-interacting protein 4, Renal carcinoma antigen NY-REN-14, DNAJA2, CPR3, HIRIP4
Tissue Specificity: Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.
Background: Ancient ubiquitous protein 1 is a protein that in humans is encoded by the AUP1 gene. The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants.
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分子量
- 42 kDa
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基因ID
- 550
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UniProt
- Q9Y679
抗原
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