Spt6 抗体 (AA 564-1639)
Quick Overview for Spt6 抗体 (AA 564-1639) (ABIN7602054)
抗原
See all Spt6 (SUPT6H) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 564-1639
-
原理
- Anti-Spt6/SUPT6H Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-Spt6/SUPT6H Antibody Picoband® (ABIN7602054). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human Spt6/SUPT6H recombinant protein (Position: A564-Q1639).
-
亚型
- IgG
-
-
anti-Suppressor of Ty 6 Homolog (SUPT6H) (AA 1500-1726) antibody
SUPT6H 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Suppressor of Ty 6 Homolog (SUPT6H) (N-Term) antibodySUPT6H 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, 豚鼠, 斑马鱼, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Suppressor of Ty 6 Homolog (SUPT6H) antibodySUPT6H 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Suppressor of Ty 6 Homolog (SUPT6H) (N-Term) antibodySUPT6H 适用: 人, 犬, Cow, 马, 兔, 豚鼠, 小鸡, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Suppressor of Ty 6 Homolog (SUPT6H) (AA 71-120) antibodySUPT6H 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, 豚鼠, 小鸡, Pig, 猴, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Suppressor of Ty 6 Homolog (SUPT6H) (AA 532-666) antibodySUPT6H 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Andrulis, E. D., Werner, J., Nazarian, A., Erdjument-Bromage, H., Tempst, P., Lis, J. T. The RNA processing exosome is linked to elongating RNA polymerase II in Drosophila. Nature 420: 837-841, 2002. 2. Chiang, P.-W., Wang, S., Smithivas, P., Song, W.-J., Ramamoorthy, S., Hillman, J., Puett, S., Van Keuren, M. L., Crombez, E., Kumar, A., Glover, T. W., Miller, D. E., Tsai, C.-H., Blackburn, C. C., Chen, X.-N., Sun, Z., Cheng, J.-F., Korenberg, J. R., Kurnit, D. M. Identification and analysis of the human and murine putative chromatin structure regulator SUPT6H and Supt6h. Genomics 34: 328-333, 1996. 3. Kaplan, C. D., Laprade, L., Winston, F. Transcription elongation factors repress transcription initiation from cryptic sites. Science 301: 1096-1099, 2003. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
-
-
- Spt6 (SUPT6H) (Suppressor of Ty 6 Homolog (SUPT6H))
-
别名
- SUPT6H
-
背景
-
Synonyms: Mediator of RNA polymerase II transcription subunit 4, Activator-recruited cofactor 36 kDa component, ARC36, Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, Vitamin D3 receptor-interacting protein complex 36 kDa component, DRIP36, MED4, ARC36, DRIP36, VDRIP, HSPC126
Background: Transcription elongation factor SPT6 is a protein that in humans is encoded by the SUPT6H gene. SUPT6H (suppressor of Ty6 homolog), also known as SPT6, SPT6H, Tat-CT2 (Tat-cotransactivator 2 protein) or emb-5 in C. elegans, is a 1,726 amino acid protein that is highly conserved from yeast to humans. Expressed ubiquitously, SUPT6H localizes to the nucleus and contains one SH2 domain and one S1 domain. SUPT6H participates in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the enhancement of transcriptional elongation by the RNA polymerase II (Pol II). SUPT6H interacts with the nuclear proteins SPT4 and SPT5, which comprise the DSIF (DRB-sensitivity-inducing factor) complex that binds RNA polymerase II, and ly regulates elongation. Via its C-terminus, SUPT6H can also interact with Histone H3. Due to alternative splicing events, three isoforms exist for SUPT6H. This antibody specifically recognizes the 230kd phosphorylated SUPT6H protein.
-
分子量
- 250 kDa
-
基因ID
- 6830
-
途径
- Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response
抗原
-
