BUB3 抗体 (AA 50-209)
Our Local Distributor
北京 101111
Quick Overview for BUB3 抗体 (AA 50-209) (ABIN7601900)
抗原
See all BUB3 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 50-209
-
原理
- Anti-BUB3 Antibody
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-BUB3 Antibody (ABIN7601900). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human BUB3 recombinant protein (Position: R50-D209).
-
亚型
- IgG
-
-
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 267-327) antibody
BUB3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 76-90), (Middle Region) antibodyBUB3 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 6-217) antibodyBUB3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) antibodyKD Validated BUB3 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB3085 unconjugated Recombinant Antibody
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (N-Term) antibodyBUB3 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibodyBUB3 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 1-328) antibodyBUB3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 201-230) antibodyBUB3 适用: Drosophila melanogaster WB 宿主: 兔 Polyclonal RB36332 unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibodyBUB3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibodyBUB3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
-
-
- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
-
别名
- BUB3
-
背景
-
Synonyms: Eosinophil cationic protein, ECP, Ribonuclease 3, RNase 3, RNASE3, ECP, RNS3
Tissue Specificity: Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level).
Background: BUB3(BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene. And it is mapped on 10q26.13. Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint(SAC), though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through ion of kinetochore localization during prometaphase to achieve biorentation. Bub3 s the localization of Bub1 at the kinetochore in order to activate the SAC. Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.
-
分子量
- 40 kDa
-
基因ID
- 9184
-
UniProt
- O43684
-
途径
- Maintenance of Protein Location
抗原
-
