BUB3 抗体 (Middle Region)
-
- 抗原 See all BUB3 抗体
- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
-
抗原表位
- AA 76-90, Middle Region
-
适用
- 人, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This BUB3 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 原理
- Rabbit IgG polyclonal antibody for Mitotic checkpoint protein BUB3(BUB3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
- 序列
- DHQLKMHDLN TDQEN
- 交叉反应 （详细）
-
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - 产品特性
-
Rabbit IgG polyclonal antibody for Mitotic checkpoint protein BUB3(BUB3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: budding uninhibited by benzimidazoles 3 homolog(yeast)
Protein Name: Mitotic checkpoint protein BUB3 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human Bub3(76-90aa DHQLKMHDLNTDQEN), identical to the related rat and mouse sequences.
- 亚型
- IgG
- Top Product
- Discover our top product BUB3 Primary Antibody
-
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) antibody
BUB3 适用: 人 WB, IF, IHC 宿主: 兔 Monoclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 267-327) antibodyBUB3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 6-217) antibodyBUB3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (N-Term) antibodyBUB3 适用: 人, 小鼠, 大鼠, Cow, 兔, 豚鼠, 马 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibodyBUB3 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibodyBUB3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 201-230) antibodyBUB3 适用: Drosophila melanogaster WB 宿主: 兔 Polyclonal RB36332 unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibodyBUB3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibodyBUB3 适用: 人, 猴 WB, IF, IHC, IP, IC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 201-300) antibodyBUB3 适用: 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
-
- 抗原
- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
- 别名
- BUB3 (BUB3 产品)
- 别名
- BUB3L antibody, hBUB3 antibody, Aa2-050 antibody, xbub3 antibody, AU019800 antibody, AU021329 antibody, AU043350 antibody, AW146323 antibody, C78067 antibody, BUB3, mitotic checkpoint protein antibody, BUB3 mitotic checkpoint protein antibody, BUB3 mitotic checkpoint protein L homeolog antibody, BUB3 antibody, Bub3 antibody, bub3.L antibody
- 背景
-
BUB3(BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene. And it is mapped on 10q26.13. Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint(SAC), though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorentation. Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC. Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.
Synonyms: Aa2-050 antibody|AU019800 antibody|AU021329 antibody|AU043350 antibody|AW146323 antibody|BUB 3 antibody|BUB 3L antibody|BUB3(budding uninhibited by benzimidazoles 3, yeast) homolog antibody|bub3 antibody|BUB3 budding uninhibited by benzimidazoles 3 homolog antibody|BUB3_HUMAN antibody|BUB3L antibody|Budding uninhibited by benomyl antibody|Budding uninhibited by benzimidazoles 3 antibody|Budding uninhibited by benzimidazoles 3 homolog(S. cerevisiae) antibody|Budding uninhibited by benzimidazoles 3 homolog(yeast) antibody|Budding uninhibited by benzimidazoles 3 homolog antibody|Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody|C78067 antibody|hBUB3 antibody|Mitotic checkpoint component antibody|Mitotic checkpoint protein bub3 antibody|OTTHUMP00000020679 antibody|OTTHUMP00000020680 antibody|WD repeat type I transmembrane protein A72.5 antibody - UniProt
- O43684
- 途径
- Maintenance of Protein Location
-
