GIT1 抗体 (AA 415-736)
Quick Overview for GIT1 抗体 (AA 415-736) (ABIN7601683)
抗原
See all GIT1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 415-736
-
原理
- Anti-GIT1 Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-GIT1 Antibody Picoband® (ABIN7601683). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human GIT1 recombinant protein (Position: D415-Q736).
-
亚型
- IgG
-
-
anti-GIT1 (GIT1) (AA 460-640) antibody
KO Validated GIT1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (AA 470-642) antibodyGIT1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (AA 533-561), (C-Term), (Tyr554) antibodyGIT1 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB15351 unconjugated
anti-GIT1 (GIT1) (Internal Region) antibodyGIT1 适用: 人, 大鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (Internal Region) antibodyGIT1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (AA 375-770) antibodyGIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 unconjugated
anti-GIT1 (GIT1) (AA 375-770) antibody (HRP)GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 HRP
anti-GIT1 (GIT1) (AA 375-770) antibody (FITC)GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 FITC
anti-GIT1 (GIT1) (AA 375-770) antibody (Biotin)GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 Biotin
anti-GIT1 (GIT1) (AA 375-770) antibody (PE)GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 PE
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Goehler, H., Lalowski, M., Stelzl, U., Waelter, S., Stroedicke, M., Worm, U., Droege, A., Lindenberg, K. S., Knoblich, M., Haenig, C., Herbst, M., Suopanki, J., and 12 others. A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease. Molec. Cell 15: 853-865, 2004. Note: Erratum: Molec. Cell 19: 287 only, 2005. 2. Gross, M. B. Personal Communication. Baltimore, Md. 7/24/2020. 3. Manabe, R., Kovalenko, M., Webb, D. J., Horwitz, A. R. GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration. J. Cell Sci. 115: 1497-1510, 2002. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
-
-
- GIT1
-
别名
- GIT1
-
背景
-
Synonyms: DNA repair protein XRCC2, X-ray repair cross-complementing protein 2, Xrcc2
Tissue Specificity: Expressed at low level in somatic tissues and at high level in testis.
Background: ARF GTPase-activating protein GIT1 is an enzyme that in humans is encoded by the GIT1 gene. G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration. GIT-1 has also been implicated in neuronal functions including synapse formation and the pathology of Huntington disease. Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains. Htt interacts ly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology.
-
分子量
- 84 kDa
-
基因ID
- 28964
-
UniProt
- Q9Y2X7
抗原
-
