CCDC99 抗体 (AA 40-605)
Quick Overview for CCDC99 抗体 (AA 40-605) (ABIN7601654)
抗原
See all CCDC99 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 40-605
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原理
- Anti-Spindly/SPDL1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Spindly/SPDL1 Antibody Picoband® (ABIN7601654). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Spindly/SPDL1 recombinant protein (Position: Q40-Q605).
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亚型
- IgG
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anti-Coiled-Coil Domain Containing 99 (CCDC99) (AA 1-605) antibody
CCDC99 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 2F4 unconjugated
anti-Coiled-Coil Domain Containing 99 (CCDC99) (AA 1-300) antibodyCCDC99 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Coiled-Coil Domain Containing 99 (CCDC99) (C-Term) antibodyCCDC99 适用: 人, 小鼠 ELISA, WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Coiled-Coil Domain Containing 99 (CCDC99) (AA 401-500) antibodyCCDC99 适用: 大鼠 ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Coiled-Coil Domain Containing 99 (CCDC99) (C-Term) antibodyCCDC99 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Coiled-Coil Domain Containing 99 (CCDC99) antibodyCCDC99 适用: 人, 小鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Coiled-Coil Domain Containing 99 (CCDC99) (AA 1-605) antibodyCCDC99 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Coiled-Coil Domain Containing 99 (CCDC99) (AA 459-488), (C-Term) antibodyCCDC99 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33449 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Barisic, M., Sohm, B., Mikolcevic, P., Wandke, C., Rauch, V., Ringer, T., Hess, M., Bonn, G., Geley, S. Spindly/CCDC99 is required for efficient chromosome congression and mitotic checkpoint regulation. Molec. Biol. Cell 21: 1968-1981, 2010. 2. Chan, Y. W., Fava, L. L., Uldschmid, A., Schmitz, M. H. A., Gerlich, D. W., Nigg, E. A., Santamaria, A. Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly. J. Cell. Biol. 185: 859-874, 2009. 3. Griffis, E. R., Stuurman, N., Vale, R. D. Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. J. Cell. Biol. 177: 1005-1015, 2007. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- CCDC99 (Coiled-Coil Domain Containing 99 (CCDC99))
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别名
- SPDL1
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背景
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Synonyms: Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FJ
Tissue Specificity: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
Background: This gene encodes a coiled-coil domain-containing protein that functions in mitotic spindle formation and chromosome segregation. The encoded protein plays a role in coordinating microtubule attachment by promoting recruitment of dynein proteins, and in mitotic checkpoint signaling.
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分子量
- 70 kDa
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基因ID
- 54908
抗原
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