NEFL 抗体 (AA 4-463)
Quick Overview for NEFL 抗体 (AA 4-463) (ABIN7601626)
抗原
See all NEFL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 4-463
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原理
- Anti-NEFL/NF-L Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-NEFL/NF-L Antibody Picoband® (ABIN7601626). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human NEFL/NF-L recombinant protein (Position: F4-A463).
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亚型
- IgG
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anti-Neurofilament, Light Polypeptide (NEFL) antibody
NEFL 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1H3 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 400-543) antibodyNEFL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) antibodyNEFL 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) antibodyNEFL 适用: 人, 大鼠, Pig, Cow, 小鸡 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal NR-4 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-360) antibodyNEFL 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3G2 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) antibodyNEFL 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 小鼠 Monoclonal 1H3 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-543) antibodyNEFL 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-284) antibodyNEFL 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 128-271) antibodyNEFL 适用: 人 IHC, IF, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-543) antibodyNEFL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunofluorescence, 5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Berciano, J., Garcia, A., Peeters, K., Gallardo, E., De Vriendt, E., Palayo-Negro, A. L., Infante, J., Jordanova, A. NEFL E396K mutation is associated with a novel dominant intermediate Charcot-Marie-Tooth disease phenotype. J. Neurol. 262: 1289-1300, 2015. 2. Hurst, J., Flavell, D., Julien, J.-P., Meijer, D., Mushynski, W., Grosveld, F. The human neurofilament gene (NEFL) is located on the short arm of chromosome 8. Cytogenet. Cell Genet. 45: 30-32, 1987. 3. Leung, C. L., Nagan, N., Graham, T. H., Liem, R. K. H. A novel duplication/insertion mutation of NEFL in a patient with Charcot-Marie-Tooth disease. Am. J. Med. Genet. 140A: 1021-1025, 2006. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NEFL (Neurofilament, Light Polypeptide (NEFL))
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别名
- NEFL
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背景
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Synonyms: Fatty acid-binding protein, liver, Fatty acid-binding protein 1, Liver-type fatty acid-binding protein, L-FABP, Squalene- and sterol-carrier protein, SCP, Z-protein, p14, Fabp1
Tissue Specificity: Expressed in lung cancers, including adenocarcinoma, squamous cell carcinoma and small-cell carcinoma. Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.
Background: Neurofilament light polypeptide (NFL), also known as neurofilament light chain, is a neurofilament protein that in humans is encoded by the NEFL gene. Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
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分子量
- 72 kDa
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基因ID
- 4747
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UniProt
- P07196
抗原
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