UPF3A 抗体 (AA 4-452)
Quick Overview for UPF3A 抗体 (AA 4-452) (ABIN7601625)
抗原
See all UPF3A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 4-452
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原理
- Anti-UPF3A Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-UPF3A Antibody Picoband® (ABIN7601625). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human UPF3A recombinant protein (Position: E4-E452).
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亚型
- IgG
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anti-UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A) (AA 274-374) antibody
UPF3A 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2E2 unconjugated
anti-UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A) (AA 327-476) antibodyUPF3A 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A) (AA 1-443) antibodyUPF3A 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A) (AA 382-475) antibodyUPF3A 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A) (AA 359-408) antibodyUPF3A 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chan, W.-K., Bhalla, A. D., Le Hir, H., Nguyen, L. S., Huang, L., Gecz, J., Wilkinson, M. F. A UPF3-mediated regulatory switch that maintains RNA surveillance. Nature Struct. Molec. Biol. 16: 747-753, 2009. 2. Gross, M. B. Personal Communication. Baltimore, Md. 2/28/2011. 3. Kim, V. N., Kataoka, N., Dreyfuss, G. Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex. Science 293: 1832-1836, 2001. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- UPF3A (UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A))
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别名
- UPF3A
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背景
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Synonyms: Disintegrin and metalloproteinase domain-containing protein 2,ADAM 2,Cancer/testis antigen 15,CT15,Fertilin subunit beta,PH-30,PH30,PH30-beta,ADAM2,FTNB,
Tissue Specificity: Expressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues.
Background: Regulator of nonsense transcripts 3A is a protein that in humans is encoded by the UPF3A gene. This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Several splice variants encoding different isoforms have been found for this gene.
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分子量
- 51 kDa
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基因ID
- 65110
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UniProt
- Q9H1J1
抗原
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