DDX21 抗体 (AA 395-660)
Quick Overview for DDX21 抗体 (AA 395-660) (ABIN7601596)
抗原
See all DDX21 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 395-660
-
原理
- Anti-DDX21 Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-DDX21 Antibody Picoband® (ABIN7601596). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human DDX21 recombinant protein (Position: E395-Q660).
-
亚型
- IgG
-
-
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21) antibody
DDX21 适用: 人, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21) (N-Term) antibodyDDX21 适用: 人, 大鼠, 马, Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21) (C-Term) antibodyDDX21 适用: 人, Cow, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21) (N-Term) antibodyDDX21 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21) (C-Term) antibodyDDX21 适用: 人, 大鼠, 马, 犬, Pig, Cow, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21) (AA 128-156), (N-Term) antibodyDDX21 适用: 人 WB 宿主: 兔 Polyclonal RB37016 unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Calo, E., Flynn, R. A., Martin, L., Spitale, R. C., Chang, H. Y., Wysocka, J. RNA helicase DDX21 coordinates transcription and ribosomal RNA processing. Nature 518: 249-253, 2015. 2. Calo, E., Gu, B., Bowen, M. E., Aryan F., Zalc, A., Liang, J., Flynn, R. A., Swigut, T., Chang, H. Y., Attardi, L. D., Wysocka, J. Tissue-selective effects of nucleolar stress and rDNA damage in developmental disorders. Nature 554: 112-117, 2018. 3. Valdez, B. C., Henning, D., Busch, R. K., Woods, K., Flores-Rozas, H., Hurwitz, J., Perlaky, L., Busch, H. A nucleolar RNA helicase recognized by autoimmune antibodies from a patient with watermelon stomach disease. Nucleic Acids Res. 24: 1220-1224, 1996. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
-
-
- DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21))
-
别名
- DDX21
-
背景
-
Synonyms: Thioredoxin, mitochondrial, MTRX, Mt-Trx, Thioredoxin-2, TXN2, TRX2
Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues.
Background: Nucleolar RNA helicase 2 is an enzyme that in humans is encoded by the DDX21 gene. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription.
-
分子量
- 86 kDa
-
基因ID
- 9188
-
途径
- SARS-CoV-2 Protein Interactome
抗原
-
