NUP214 抗体 (AA 36-374)
Quick Overview for NUP214 抗体 (AA 36-374) (ABIN7601473)
抗原
See all NUP214 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 36-374
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原理
- Anti-NUP214 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-N Antibody Picoband® (ABIN7601473). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human NUP214 recombinant protein (Position: K36-E374).
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亚型
- IgG
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anti-Nucleoporin 214kDa (NUP214) (AA 28-238) antibody
NUP214 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 214kDa (NUP214) (AA 601-868) antibodyNUP214 适用: 小鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 214kDa (NUP214) (AA 1-97) antibodyNUP214 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Nucleoporin 214kDa (NUP214) (AA 750-1030) antibodyNUP214 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 214kDa (NUP214) antibodyNUP214 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 214kDa (NUP214) (C-Term) antibodyNUP214 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Kraemer D, Wozniak RW, Blobel G, Radu A (Feb 1994). "The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm". Proceedings of the National Academy of Sciences of the United States of America. 91 (4): 1519-23. 2. von Lindern M, Poustka A, Lerach H, Grosveld G (Aug 1990). "The (6,9) chromosome translocation, associated with a specific subtype of acute nonlymphocytic leukemia, leads to aberrant transcription of a target gene on 9q34". Molecular and Cellular Biology. 10 (8): 4016-26. 3. Napetschnig J, Blobel G, Hoelz A (2007). "Crystal structure of the N-terminal domain of the human protooncogene Nup214/CAN". Proc. Natl. Acad. Sci. U.S.A. 104 (6): 1783-8. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NUP214 (Nucleoporin 214kDa (NUP214))
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别名
- NUP214
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背景
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Synonyms: Nuclear pore complex protein Nup214, 214 kDa nucleoporin, Nucleoporin Nup214, Protein CAN, NUP214, CAIN, CAN, KIAA0023
Tissue Specificity: Expressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development.
Background: Nucleoporin 214 (Nup2014) is a protein that in humans is encoded by the NUP214 gene. The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t (6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
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分子量
- 280 kDa
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基因ID
- 8021
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UniProt
- P35658
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途径
- Protein targeting to Nucleus, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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