ENOS 抗体 (AA 34-1153)
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北京 101111
Quick Overview for ENOS 抗体 (AA 34-1153) (ABIN7601375)
抗原
See all ENOS (NOS3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 34-1153
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原理
- Anti-eNOS/NOS3 Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-eNOS/NOS3 Antibody (ABIN7601375). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human eNOS/NOS3 recombinant protein (Position: P34-Q1153).
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亚型
- IgG
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anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pSer1177) antibody
NOS3 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 1105-1202) antibodyNOS3 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pSer1177) antibodyNOS3 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (C-Term) antibodyNOS3 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 1-120) antibodyNOS3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 1156-1183) antibodyNOS3 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pThr495) antibodyNOS3 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 756-888) antibodyNOS3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 61-160) antibodyNOS3 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 1D12 unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (Thr180), (Tyr182) antibodyNOS3 适用: 人, 大鼠, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
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别名
- NOS3
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背景
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Synonyms: Nitric oxide synthase, endothelial, Constitutive NOS, Cnos, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII, NOS3
Tissue Specificity: Platelets, placenta, liver and kidney.
Background: NOS3(Nitric Oxide Synthase 3), also called ENOS, anitric oxide synthasethat generatesNOinblood vesselsand is involved with regulatingvascular toneby inhibitingsmooth musclecontraction and plateletaggregation. The NOS3 gene is mapped on 7q36.1. Variations in this gene are associated with susceptibility tocoronary spasm. Fulton et al.(1999) concluded the eNOS is an AKT substrate linking signal transduction by AKT to the release of the gaseous second messenger nitric oxide. AKT mediates the activation of eNOS, leading to increased nitric oxide production. Inhibition of the PI3K AKT pathway or mutation of the AKT site on eNOS protein at serine-1177 attenuated the serine phosphorylation and prevented the activation of eNOS. RT-PCR analysis showed that expression ofNOS3in human umbilical vein endothelial cells (HUVECs) and human aortic vascular smooth muscle cells (HAOVSMCs) was inversely proportional to that of NOS3AS.
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分子量
- 140 kDa
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基因ID
- 4846
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UniProt
- P29474
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途径
- ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
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