HIP1R 抗体 (AA 316-734)
Quick Overview for HIP1R 抗体 (AA 316-734) (ABIN7601290)
抗原
See all HIP1R 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 316-734
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原理
- Anti-HIP1R Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-HIP1R Antibody Picoband® (ABIN7601290). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human HIP1R recombinant protein (Position: E316-E734).
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亚型
- IgG
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anti-Huntingtin Interacting Protein 1 Related (HIP1R) (AA 1-1068) antibody
HIP1R 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Huntingtin Interacting Protein 1 Related (HIP1R) (AA 1-1068) antibodyHIP1R 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Huntingtin Interacting Protein 1 Related (HIP1R) (AA 969-1066) antibodyHIP1R 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E10 unconjugated
anti-Huntingtin Interacting Protein 1 Related (HIP1R) (AA 304-487) antibodyHIP1R 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Huntingtin Interacting Protein 1 Related (HIP1R) (N-Term) antibodyHIP1R 适用: 人 WB, IHC, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Huntingtin Interacting Protein 1 Related (HIP1R) (AA 771-1012) antibodyHIP1R 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Huntingtin Interacting Protein 1 Related (HIP1R) (AA 98-126), (N-Term) antibodyHIP1R 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB18066 unconjugated
anti-Huntingtin Interacting Protein 1 Related (HIP1R) (AA 300-600) antibodyHIP1R 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Huntingtin Interacting Protein 1 Related (HIP1R) (AA 105-133), (N-Term) antibodyHIP1R 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bradley, S. V., Hyun, T. S., Oravecz-Wilson, K. I., Li, L., Waldorff, E. I., Ermilov, A. N., Goldstein, S. A., Zhang, C. X., Drubin, D. G., Varela, K., Parlow, A., Dlugosz, A. A., Ross, T. S. Degenerative phenotypes caused by the combined deficiency of murine HIP1 and HIP1r are rescued by human HIP1. Hum. Molec. Genet. 16: 1279-1292, 2007. 2. Chopra, V. S., Metzler, M., Rasper, D. M., Engqvist-Goldstein, A. E. Y., Singaraja, R., Gan, L., Fichter, K. M., McCutcheon, K., Drubin, D., Nicholson, D. W., Hayden, M. R. HIP12 is a non-proapoptotic member of a gene family including HIP1, an interacting protein with huntingtin. Mammalian Genome 11: 1006-1015, 2000. 3. Engqvist-Goldstein, A. E. Y., Kessels, M. M., Chopra, V. S., Hayden, M. R., Drubin, D. G. An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles. J. Cell Biol. 147: 1503-1518, 1999. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- HIP1R (Huntingtin Interacting Protein 1 Related (HIP1R))
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别名
- HIP1R
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背景
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Synonyms: Centriolar coiled-coil protein of 110 kDa, Centrosomal protein of 110 kDa, CP110, Cep110, CCP110, CEP110, CP110, KIAA0419
Tissue Specificity: Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.
Background: Huntingtin-interacting protein 1-related protein is a protein that in humans is encoded by the HIP1R gene. Huntingtin-interacting protein 1-related protein, or HIP1R, was identified on the basis of its structural homology with Huntingtin-interacting protein 1, or HIP1. Based on its domain structure, HIP1R is a putative endocytosis-related protein. Knockdown of HIP1R impairs the endocytosis of activated epidermal growth factor receptor (EGFR) and the consequent activation of the downstream ERK and Akt proteins. Additionally, HIP1R is a component of the clathrin-coated pits and vesicles, which in part links the endocytic machinery to the actin cytoskeleton. It binds to 3-phosphoinositides via ENTH domains to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. HIP1R deficiency significantly reduces the expression of the ionotropic glutamate receptor GluA1, GluN2A, and GluN2B subunits, but not the GABAA receptor α1 subunit. Knockdown of HIP1R reduces the amplitude and frequency of the miniature excitatory postsynaptic current, but not of the miniature inhibitory postsynaptic curren. HIP1R has been identified as a protein that can target histone deacetylase-3-mediated neurodegeneration, along with other proteins like NPTX1, NFL, TEX10, and TGFFG.
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分子量
- 120 kDa
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基因ID
- 9026
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UniProt
- O75146
抗原
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