KRT20 抗体 (AA 29-431)
Quick Overview for KRT20 抗体 (AA 29-431) (ABIN7601133)
抗原
See all KRT20 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 29-431
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原理
- Anti-Cytokeratin 20/Krt20 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Cytokeratin 20/Krt20 Antibody Picoband® (ABIN7601133). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived mouse Cytokeratin 20/Krt20 recombinant protein (Position: R29-V431).
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亚型
- IgG
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anti-Keratin 20 (KRT20) antibody
KRT20 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 20 (KRT20) (AA 1-424) antibodyKRT20 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2G3-1C8 unconjugated
anti-Keratin 20 (KRT20) (AA 245-424) antibodyKRT20 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal KRT20-1992 unconjugated
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal KRT20-1991 unconjugated
anti-Keratin 20 (KRT20) (AA 1-266) antibodyKRT20 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4A2 unconjugated
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal KRT20-3129R unconjugated Recombinant Antibody
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal KRT20-3145 unconjugated
anti-Keratin 20 (KRT20) (AA 1-150) antibodyKRT20 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2C7B9 unconjugated
anti-Keratin 20 (KRT20) antibodyKRT20 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 1F10 unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human, Rat
Flow Cytometry (Fixed)|1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Calnek, D., Quaroni, A. Differential localization by in situ hybridization of distinct keratin mRNA species during intestinal epithelial cell development and differentiation. Differentiation 53: 95-104, 1993. 2. Moll, R., Schiller, D. L., Franke, W. W. Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J. Cell Biol. 111: 567-580, 1990. 3. Rogers, M. A., Langbein, L., Winter, H., Ehmann, C., Praetzel, S., Korn, B., Schweizer, J. Characterization of a cluster of human high/ultrahigh sulfur keratin-associated protein genes embedded in the type I keratin gene domain on chromosome 17q12-21. J. Biol. Chem. 276: 19440-19451, 2001. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- KRT20 (Keratin 20 (KRT20))
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别名
- Krt20
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背景
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Synonyms: Keratin, type I cytoskeletal 20, Cytokeratin-20, CK-20, Keratin-20, K20, Krt20
Tissue Specificity: Expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Also expressed in epithelial cells of bladder, ileum and stomach and at lower levels in pancreas and earskin. The phosphorylated form is nearly exclusively expressed in goblet cells of the small intestine and in the lumen-proximal cells of the colon (at protein level). Also expressed in jejunum and duodenum.
Background: Keratin 20, often abbreviated CK20, is a protein that in humans is encoded by the KRT20 gene. This gene encodes a member of the keratin protein family and is found within a cluster of cytokeratin genes on chromosome 11. Keratins are cytoskeletal proteins that are preferentially expressed in epithelial cells. The encoded protein may help maintain intermediate filament organization in intestinal epithelium. Phosphorylation of this protein may also influence mucin secretion in the small intestine.
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分子量
- 48 kDa
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基因ID
- 66809
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UniProt
- Q9D312
抗原
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