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Ki-67 抗体 (AA 2860-3256)

This anti-Ki-67 antibody is a 小鼠 单克隆 antibody detecting Ki-67 in IHC, WB, FACS, IF 和 ICC. Suitable for 人.
产品编号 ABIN7601101
发货至: 中国

Quick Overview for Ki-67 抗体 (AA 2860-3256) (ABIN7601101)

抗原

See all Ki-67 (MKI67) 抗体
Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Ki-67 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

克隆位点

5C7
  • 抗原表位

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    AA 2860-3256

    原理

    Anti-Ki67 Antibody Picoband® (monoclonal, 5C7)

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-Ki67 Antibody Picoband® (monoclonal, 5C7) (ABIN7601101). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E. coli-derived human Ki67 recombinant protein (Position: K2860-I3256).

    亚型

    IgG2b
  • 应用备注

    Western blot, 0.25-0.5 μg/mL, Human
    Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. Fonatsch, C., Duchrow, M., Rieder, H., Schluter, C., Gerdes, J. Assignment of the human Ki-67 gene (MKI67) to 10q25-qter. Genomics 11: 476-477, 1991. 2. Schonk, D. M., Kuijpers, H. J. H., vanDrunen, E., vanDalen, C. H., Geurts van Kessel, A. H. M., Verheijen, R., Ramaekers, F. C. S. Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10. Hum. Genet. 83: 297-299, 1989. 3. Traut, W., Scholzen, T., Winking, H., Kubbutat, M. H. G., Gerdes, J. Assignment of the murine Ki-67 gene (Mki67) to chromosome band 7F3-F5 by in situ hybridization. Cytogenet. Cell Genet. 83: 12-13, 1998.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • 抗原

    Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

    别名

    MKI67

    背景

    Synonyms: Tuberin, Tuberous sclerosis 2 protein, TSC2, TSC4

    Tissue Specificity: Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.

    Background: Ki-67(Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

    分子量

    358 kDa

    基因ID

    4288

    UniProt

    P46013

    途径

    Glycosaminoglycan Metabolic Process
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