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NeuN 抗体 (AA 28-304)

This anti-NeuN antibody is a 兔 多克隆 antibody detecting NeuN in WB, IHC, ELISA 和 IF. Suitable for 小鼠 和 大鼠.
产品编号 ABIN7601068
发货至: 中国

Quick Overview for NeuN 抗体 (AA 28-304) (ABIN7601068)

抗原

See all NeuN (RBFOX3) 抗体
NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

适用

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  • 16
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  • 1
小鼠, 大鼠

宿主

  • 46
  • 15
  • 1

克隆类型

  • 35
  • 27
多克隆

标记

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  • 2
  • 1
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This NeuN antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
  • 抗原表位

    • 16
    • 5
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    AA 28-304

    原理

    Anti-NeuN/Rbfox3 Antibody Picoband®

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-NeuN/Rbfox3 Antibody Picoband® (ABIN7601068). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived mouse NeuN/Rbfox3 recombinant protein (Position: H28-D304).

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.25 μg/mL, Mouse, Rat
    Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
    Immunofluorescence, 5 μg/mL, Rat
    ELISA, 0.1-0.5 μg/mL, -
    1. Dredge, B. K., Jensen, K. B. NeuN/Rbfox3 nuclear and cytoplasmic isoforms differentially regulate alternative splicing and nonsense-mediated decay of Rbfox2. PLoS One 6: e21585, 2011. Note: Electronic Article. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 6/22/2016. 3. Kim, K. K., Adelstein, R. S., Kawamoto, S. Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors. J. Biol. Chem. 284: 31052-31061, 2009.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • 抗原

    NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

    别名

    Rbfox3

    背景

    Synonyms: Solute carrier family 2, facilitated glucose transporter member 6, Glucose transporter type 6, GLUT-6, Glucose transporter type 9, GLUT-9, SLC2A6, GLUT9

    Tissue Specificity: Highly expressed in brain, spleen and peripheral blood leukocytes.

    Background: RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3) is a protein that in humans is encoded by the RBFOX3 gene. This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

    分子量

    46-55 kDa

    基因ID

    52897
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