EZH1 抗体 (AA 27-485)
Quick Overview for EZH1 抗体 (AA 27-485) (ABIN7601019)
抗原
See all EZH1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 27-485
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原理
- Anti-EZH1 Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins
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产品特性
- Anti-EZH1 Antibody Picoband® (ABIN7601019). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human EZH1 recombinant protein (Position: R27-E485).
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Abel, K. J., Brody, L. C., Valdes, J. M., Erdos, M. R., McKinley, D. R., Castilla, L. H., Merajver, S. D., Couch, F. J., Friedman, L. S., Ostermeyer, E. A., Lynch, E. D., King, M.-C., Welcsh, P. L., Osborne-Lawrence, S., Spillman, M., Bowcock, A. M., Collins, F. S., Weber, B. L. Characterization of EZH1, a human homolog of Drosophila enhancer of zeste near BRCA1. Genomics 37: 161-171, 1996. 2. Laible, G., Haynes, A. R., Lebersorger, A., O'Carroll, D., Mattei, M.-G., Denny, P., Brown, S. D. M., Jenuwein, T. The murine polycomb-group genes Ezh1 and Ezh2 map close to Hox gene clusters on mouse chromosomes 11 and 6. Mammalian Genome 10: 311-314, 1999. 3. Maier, V. K., Feeney, C. M., Taylor, J. E., Creech, A. L., Qiao, J. W., Szanto, A., Das, P. P., Chevrier, N., Cifuentes-Rojas, C., Orkin, S. H., Carr, S. A., Jaffe, J. D., Mertins, P., Lee, J. T. Functional proteomic analysis of repressive histone methyltransferase complexes reveals ZNF518B as a G9A regulator. Molec. Cell. Proteomics 14: 1435-1446, 2015. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- EZH1 (Enhancer of Zeste Homolog 1 (EZH1))
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别名
- EZH1
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背景
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Synonyms: Heat shock protein beta-8,HspB8,Alpha-crystallin C chain,E2-induced gene 1 protein,Protein kinase H11,Small stress protein-like protein HSP22,HSPB8,CRYAC, E2IG1, HSP22,PP1629,
Tissue Specificity: Predominantly expressed in skeletal muscle and heart. .
Background: Histone-lysine N-methyltransferase EZH1 is an enzyme that in humans is encoded by the EZH1 gene. EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity.
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分子量
- 110 kDa, 60 kDa
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基因ID
- 2145
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UniProt
- Q92800
抗原
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