BRSK1 抗体 (AA 269-321)
Quick Overview for BRSK1 抗体 (AA 269-321) (ABIN7600990)
抗原
See all BRSK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 269-321
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原理
- Anti-BRSK1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-BRSK1 Antibody Picoband® (ABIN7600990). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human BRSK1 recombinant protein (Position: E269-E321).
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亚型
- IgG
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anti-BR Serine/threonine Kinase 1 (BRSK1) (AA 310-510) antibody
BRSK1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-BR Serine/threonine Kinase 1 (BRSK1) (AA 426-591) antibodyBRSK1 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-BR Serine/threonine Kinase 1 (BRSK1) (AA 355-384) antibodyBRSK1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB40234 unconjugated
anti-BR Serine/threonine Kinase 1 (BRSK1) (Internal Region) antibodyBRSK1 适用: 人, 小鼠 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-BR Serine/threonine Kinase 1 (BRSK1) (AA 654-684), (C-Term) antibodyBRSK1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB5415 unconjugated
anti-BR Serine/threonine Kinase 1 (BRSK1) (C-Term) antibodyBRSK1 适用: 人, 小鼠, 大鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kishi, M., Pan, Y. A., Crump, J. G., Sanes, Y. R. Mammalian SAD kinases are required for neuronal polarization. Science 307: 929-932, 2005. 2. Nagase, T., Nakayama, M., Nakajima, D., Kikuno, R., Ohara, O. Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 8: 85-95, 2001. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- BRSK1 (BR Serine/threonine Kinase 1 (BRSK1))
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别名
- BRSK1
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背景
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Synonyms: Tricarboxylate transport protein, mitochondrial, Citrate transport protein, CTP, Solute carrier family 25 member 1, Tricarboxylate carrier protein, SLC25A1, SLC20A3
Tissue Specificity: Detected in brain. Detected at very much lower levels in heart, lung, placenta and skeletal muscle. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
Background: BR serine/threonine kinase 1 is an enzyme that in humans is encoded by the BRSK1 gene. BRSK1 was initially identified as a mammalian homolog to the fission yeast S. pombe Cdr2, a mitosis-regulatory kinase and also shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK1 is unbiquitously expressed, with highest levels of expression in the brain and testes. Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest. Overexpression of this protein leads to the G2/M arrest in HeLa S2 cells and UV-induced G2/M arrest could be partially abrogated by reduced expression of BRSK1 through the use of siRNA, indicating its role in DNA damage checkpoint function. More recently, it has been shown that both BRSK1 and the related protein BRSK2 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. At least two isoforms of BRSK1 are known to exist.
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分子量
- 90 kDa
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基因ID
- 84446
抗原
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