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BUB1B 抗体 (AA 26-448)

This anti-BUB1B antibody is a 兔 多克隆 antibody detecting BUB1B in WB, IHC, ELISA, IF, FACS 和 ICC. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN7600962
发货至: 中国

Quick Overview for BUB1B 抗体 (AA 26-448) (ABIN7600962)

抗原

See all BUB1B 抗体
BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

适用

  • 99
  • 55
  • 31
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  • 1
人, 小鼠, 大鼠

宿主

  • 75
  • 24
  • 4

克隆类型

  • 57
  • 46
多克隆

标记

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  • 1
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This BUB1B antibody is un-conjugated

应用范围

  • 86
  • 43
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • 抗原表位

    • 15
    • 8
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    AA 26-448

    原理

    Anti-BubR1/BUB1B Antibody

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-BubR1/BUB1B Antibody (ABIN7600962). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human BubR1/BUB1B recombinant protein (Position: K26-E448).

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.25 μg/mL, Human
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

    别名

    BUB1B

    背景

    Synonyms: Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, MAD3/BUB1-related protein kinase, Hbubr1, Mitotic checkpoint kinase MAD3L, Protein SSK1, BUB1B, BUBR1, MAD3L, SSK1

    Tissue Specificity: Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.

    Background: Mitotic checkpoint serine/threonine-protein kinase BUB1 beta?is an?enzyme?that in humans is encoded by the?BUB1B?gene. This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

    分子量

    120-130 kDa

    基因ID

    701

    UniProt

    O60566
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