BUB1B 抗体 (AA 26-448)
Quick Overview for BUB1B 抗体 (AA 26-448) (ABIN7600961)
抗原
See all BUB1B 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 26-448
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原理
- Anti-BubR1/BUB1B Antibody Picoband® (monoclonal, 8B3)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-BubR1/BUB1B Antibody Picoband® (monoclonal, 8B3) (ABIN7600961). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human BubR1/BUB1B recombinant protein (Position: K26-E448).
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亚型
- IgG2b
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anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-130) antibody
BUB1B 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2G5 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibodyBUB1B 适用: 人, 大鼠, 猴, 犬 WB, IF, FACS 宿主: 小鼠 Monoclonal 6E3 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibodyBUB1B 适用: 人, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 11G2 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-130) antibodyBUB1B 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2G9 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 230-394) antibodyBUB1B 适用: 人 IHC, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 274-525) antibodyBUB1B 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-130) antibodyBUB1B 适用: 人 WB, IHC, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2G5 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (C-Term) antibodyBUB1B 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibodyBUB1B 适用: 人, 小鼠 WB, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8G1 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 751-850) antibodyBUB1B 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Davenport JW, Fernandes ER, Harris LD, Neale GA, Goorha R (March 1999). "The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner". Genomics. 55 (1): 113-7. 2. Guo Y, Kim C, Ahmad S, Zhang J, Mao Y (July 2012). "CENP-E--dependent BubR1 autophosphorylation enhances chromosome alignment and the mitotic checkpoint". J. Cell Biol. 198 (2): 205-217. 3. Baker DJ, Dawlaty MM, Wijshake T, Jeganathan KB, Malureanu L, van Ree JH, Crespo-Diaz R, Reyes S, Seaburg L, Shapiro V, Behfar A, Terzic A, van de Sluis B, van Deursen JM (December 2012). "Increased expression of BubR1 protects against aneuploidy and cancer and extends healthy lifespan". Nat. Cell Biol. 15 (1): 96-102. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))
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别名
- BUB1B
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背景
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Synonyms: Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, MAD3/BUB1-related protein kinase, Hbubr1, Mitotic checkpoint kinase MAD3L, Protein SSK1, BUB1B, BUBR1, MAD3L, SSK1
Tissue Specificity: Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
Background: Mitotic checkpoint serine/threonine-protein kinase BUB1 betais anenzymethat in humans is encoded by theBUB1Bgene. This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
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分子量
- 130 kDa
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基因ID
- 701
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UniProt
- O60566
抗原
-
