UBE2M 抗体 (AA 25-154)
Quick Overview for UBE2M 抗体 (AA 25-154) (ABIN7600885)
抗原
See all UBE2M (ube2m) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 25-154
-
原理
- Anti-UBC12/UBE2M Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-UBC12/UBE2M Antibody Picoband® (ABIN7600885). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human UBC12/UBE2M recombinant protein (Position: K25-Q154).
-
亚型
- IgG
-
-
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (N-Term) antibody
ube2m 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-183) antibodyube2m 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3C4 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (Internal Region) antibodyube2m 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 11-28) antibodyube2m 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) antibodyube2m 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 169-180) antibodyube2m 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (N-Term) antibodyube2m 适用: 人, 小鼠, 非洲爪蟾, 大鼠, 犬, Cow, 豚鼠, 马, 猴, 兔 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Huang, D. T., Hunt, H. W., Zhuang, M., Ohi, M. D., Holton, J. M., Schulman, B. A. Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity. Nature 445: 394-398, 2007. 2. Osaka, F., Kawasaki, H., Aida, N., Saeki, M., Chiba, T., Kawashima, S., Tanaka, K., Kato, S. A new NEDD8-ligating system for cullin-4A. Genes Dev. 12: 2263-2268, 1998. 3. Scott, D. C., Monda, J. K., Bennett, E. J., Harper, J. W., Schulman, B. A. N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex. Science 334: 674-678, 2011. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
-
-
- UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
-
别名
- UBE2M
-
背景
-
Synonyms: BAG family molecular chaperone regulator 5,BAG-5,Bcl-2-associated athanogene 5,BAG5,KIAA0873,
Tissue Specificity: Expressed in all tissues examined with lower levels in brain and testis.
Background: NEDD8-conjugating enzyme Ubc12 is a protein that in humans is encoded by the UBE2M gene. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.
-
分子量
- 21 kDa
-
基因ID
- 9040
-
UniProt
- P61081
抗原
-
