UBE2M 抗体 (AA 169-180)
(2 references)-
- 抗原 See all UBE2M (ube2m) 抗体
- UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
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抗原表位
- AA 169-180
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This UBE2M antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应
- Chimpanzee, 犬, Frog, 人, 小鼠, 大鼠
- 纯化方法
- UBC12 Antibody is produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human UBC12 protein. However, 100% homology is on record for this protein from human, chimpanzee, frog, mouse, rat and dog as indicated by BLAST analysis. Reactivity with UBC12 proteins from other sources is not known.
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 169-180 of Human UBC12 protein.
Immunogen Type: Peptide
- 亚型
- IgG
- Top Product
- Discover our top product ube2m Primary Antibody
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anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (N-Term) antibody
ube2m 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-183) antibodyube2m 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3C4 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (Internal Region) antibodyube2m 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 11-28) antibodyube2m 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) antibodyube2m 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (N-Term) antibodyube2m 适用: 人, 小鼠, 大鼠, 非洲爪蟾, 犬, 猴, 兔, Cow, 豚鼠, 马 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: UBC12 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to UBC12 by western blotting in the appropriate cell lysate or extract.
ELISA Dilution: 1:2,000 - 1:10,000
Western Blot Dilution: 1:500 - 1:2,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01 % (w/v) Sodium AzideStabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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: "Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression." in: Molecular cell, Vol. 69, Issue 5, pp. 773-786.e6, (2019) (PubMed).
: "Alphavirus production is inhibited in neurofibromin 1-deficient cells through activated RAS signalling." in: Virology, Vol. 377, Issue 1, pp. 133-42, (2008) (PubMed).
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: "Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression." in: Molecular cell, Vol. 69, Issue 5, pp. 773-786.e6, (2019) (PubMed).
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- 抗原
- UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
- 别名
- UBC12 (ube2m 产品)
- 别名
- DDBDRAFT_0204633 antibody, DDBDRAFT_0238042 antibody, DDB_0204633 antibody, DDB_0238042 antibody, UBC-RS2 antibody, UBC12 antibody, hUbc12 antibody, 2510040H03Rik antibody, Ubc-rs2 antibody, ubiquitin conjugating enzyme E2M antibody, ubiquitin-conjugating enzyme E2M antibody, ubiquitin conjugating enzyme E2 M antibody, NEDD8-conjugating enzyme antibody, ubiquitin conjugating enzyme E2M S homeolog antibody, ube2m antibody, LOC692934 antibody, UBE2M antibody, Ube2m antibody, ube2m.S antibody
- 背景
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Synonyms: rabbit anti-UBC12 antibody, UBC-12, UBC 12, NEDD-8, NEDD 8, NEDD8 carrier protein antibody, NEDD8 conjugating enzyme Ubc12 antibody, NEDD8 protein ligase antibody, UBE2M antibody, Ubiquitin conjugating enzyme E2 M antibody
Background: UBC12 (also known as Ubiquitin-conjugating enzyme E2M, Ubiquitin-protein ligase M, Ubiquitin carrier protein M, and Nedd8-conjugating enzyme Ubc12) is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBC12 is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/cullin.
Gene Name: UBE2M
- 基因ID
- 9040, 4507791
- UniProt
- P61081
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