RENT2/UPF2 抗体 (AA 200-651)
Quick Overview for RENT2/UPF2 抗体 (AA 200-651) (ABIN7600567)
抗原
See all RENT2/UPF2 (UPF2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 200-651
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原理
- Anti-hUPF2/RENT2/UPF2 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-hUPF2/RENT2/UPF2 Antibody Picoband® (ABIN7600567). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human hUPF2/RENT2/UPF2 recombinant protein (Position: K200-H651).
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亚型
- IgG
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anti-UPF2 Regulator of Nonsense Transcripts 2 (UPF2) (AA 1153-1272) antibody
UPF2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-UPF2 Regulator of Nonsense Transcripts 2 (UPF2) antibodyUPF2 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-UPF2 Regulator of Nonsense Transcripts 2 (UPF2) (AA 1153-1272) antibodyUPF2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-UPF2 Regulator of Nonsense Transcripts 2 (UPF2) antibodyUPF2 适用: 人, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-UPF2 Regulator of Nonsense Transcripts 2 (UPF2) (AA 51-100) antibodyUPF2 适用: 人, 大鼠, 小鼠, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-UPF2 Regulator of Nonsense Transcripts 2 (UPF2) (N-Term) antibodyUPF2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-UPF2 Regulator of Nonsense Transcripts 2 (UPF2) antibodyUPF2 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-UPF2 Regulator of Nonsense Transcripts 2 (UPF2) (AA 630-656) antibodyUPF2 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB21963 unconjugated
anti-UPF2 Regulator of Nonsense Transcripts 2 (UPF2) (AA 633-660), (Middle Region) antibodyUPF2 适用: 人 WB, FACS, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Johnson, J. L., Stoica, L., Liu, Y., Zhu, P. J., Bhattacharya, A., Buffington, S. A., Huq, R., Eissa, N. T., Larsson, O., Porse, B. T., Domingo, D., Nawaz, U., and 19 others. Inhibition of Upf2-dependent nonsense-mediated decay leads to behavioral and neurophysiological abnormalities by activating the immune response. Neuron 104: 665-679, 2019. 2. Lykke-Andersen, J., Shu, M.-D., Steitz, J. A. Human Upf proteins target an mRNA for nonsense-mediated decay when bound downstream of a termination codon. Cell 103: 1121-1131, 2000. 3. Mendell, J. T., ap Rhys, C. M. J., Dietz, H. C. Separable roles for rent1/hUpf1 in altered splicing and decay of nonsense transcripts. Science 298: 419-371, 2002. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- RENT2/UPF2 (UPF2) (UPF2 Regulator of Nonsense Transcripts 2 (UPF2))
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别名
- UPF2
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背景
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Synonyms: Protocadherin-15, PCDH15, USH1F
Tissue Specificity: Expressed in brain, lung, kidney, spleen and testis. Found also in the inner and outer synaptic layers, and the nerve fiber layer in adult and fetal retinas. Found in the supporting cells, outer sulcus cells and spiral ganglion of fetal cochlea. Expressed in cytotoxic tumor-derived T- and NK-cell lines as well as biopsies of nasal NK/T-cell lymphomas. Not detected in normal or in vitro activated peripheral blood cells, CD4 or CD8 lymphocytes or NK cells. Isoform 3 is expressed in brain, heart, cerebellum and kidney. CD1 isoforms, such as isoform 1, have a limited pattern of expression and is detected in testis, retina and cochlea. CD2 isoforms, such as isoforms 4 and 5, are expressed in heart, kidney, thymus, spleen, testis, retina and cochlea. CD3 isoforms, such as isoform 6, are widely expressed.
Background: Regulator of nonsense transcripts 2 is a protein that in humans is encoded by the UPF2 gene. This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene, both variants encode the same protein.
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分子量
- 170 kDa
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基因ID
- 26019
抗原
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