Hexokinase 1 抗体 (AA 17-323)
Quick Overview for Hexokinase 1 抗体 (AA 17-323) (ABIN7600259)
抗原
See all Hexokinase 1 (HK1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 17-323
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原理
- Anti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 4B7)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 4B7) (ABIN7600259). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Hexokinase 1/HK1 recombinant protein (Position: D17-R323).
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亚型
- IgG2a
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anti-Hexokinase 1 (HK1) (AA 413-540) antibody
HK1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Hexokinase 1 (HK1) antibodyKO Validated HK1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Monoclonal unconjugated
anti-Hexokinase 1 (HK1) (AA 20-300) antibodyHK1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Hexokinase 1 (HK1) (AA 1-916) antibodyHK1 适用: 人 WB, IP, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Hexokinase 1 (HK1) antibodyHK1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 3A10 unconjugated
anti-Hexokinase 1 (HK1) (AA 78-108), (N-Term) antibodyHK1 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB3921-RB3922 unconjugated
anti-Hexokinase 1 (HK1) antibodyHK1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7A7 unconjugated
anti-Hexokinase 1 (HK1) antibodyHK1 适用: 人 WB, ICC, IF, IHC (p), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Hexokinase 1 (HK1) (AA 1-917), (N-Term) antibodyHK1 适用: 人 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 4D7 unconjugated
anti-Hexokinase 1 (HK1) (AA 80-215) antibodyHK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Andreoni, F., Ruzzo, A., Magnani, M. Structure of the 5-prime region of the human hexokinase type I (HKI) gene and identification of an additional testis-specific HKI mRNA. Biochim. Biophys. Acta 1493: 19-26, 2000. 2. Bianchi, M., Crinelli, R., Serafini, G. Giammarini, C., Magnani, M. Molecular bases of hexokinase deficiency. Biochim. Biophys. Acta 1360: 211-221, 1997. 3. Bianchi, M., Magnani, M. Hexokinase mutations that produce nonspherocytic hemolytic anemia. Blood Cells Mol. Dis. 21: 2-8, 1995. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Hexokinase 1 (HK1)
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别名
- HK1
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背景
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Synonyms: Hexokinase-1, Brain form hexokinase, Hexokinase type I, HK I, HK1
Tissue Specificity: Ubiquitous.
Background: Hexokinase-1 (HK1) is an enzyme that in humans is encoded by the HK1 gene on chromosome 10. It is mapped to 10q22.1. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
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分子量
- 120 kDa
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基因ID
- 3098
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UniProt
- P19367
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途径
- Carbohydrate Homeostasis, Warburg Effect
抗原
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