CDC45 抗体 (AA 166-431)
Quick Overview for CDC45 抗体 (AA 166-431) (ABIN7600230)
抗原
See all CDC45 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 166-431
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原理
- Anti-CDC45L Picoband® Antibody (monoclonal, 6H6)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-CDC45L Picoband® Antibody (monoclonal, 6H6) (ABIN7600230). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human CDC45L recombinant protein (Position: E166-A431).
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亚型
- IgG1
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anti-Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (AA 1-270) antibody
CDC45 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (AA 1-566) antibodyCDC45 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3F11-1F3 unconjugated
anti-Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (AA 1-566) antibodyCDC45 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (AA 477-566) antibodyCDC45 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4C2 unconjugated
anti-Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (AA 1-566) antibodyCDC45 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (AA 1-566) antibodyCDC45 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (C-Term) antibodyCDC45 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (Center) antibodyCDC45 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (AA 236-263) antibodyCDC45 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB23958 unconjugated
anti-Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (AA 485-566) antibodyCDC45 适用: 小鼠, 大鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. "Entrez Gene: CDC45L CDC45 cell division cycle 45-like (S. cerevisiae)". 2. McKie JM, Wadey RB, Sutherland HF, Taylor CL, Scambler PJ (Sep 1998). " selection of conserved cDNAs from the DiGeorge critical region: isolation of a novel CDC45-like gene". Genome Res. 8 (8): 834-41. 3. Saha P, Thome KC, Yamaguchi R, Hou Z, Weremowicz S, Dutta A (Aug 1998). "The human homolog of Saccharomyces cerevisiae CDC45". J. Biol. Chem. 273 (29): 18205-9. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))
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别名
- CDC45
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背景
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Synonyms: Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18, CYK18, PIG46
Tissue Specificity: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Background: CDC45 is a protein that in humans is encoded by the CDC45L gene. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.
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分子量
- 66 kDa
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基因ID
- 8318
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UniProt
- O75419
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途径
- Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
抗原
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