SRF 抗体 (AA 163-508)
Quick Overview for SRF 抗体 (AA 163-508) (ABIN7600213)
抗原
See all SRF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 163-508
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原理
- Anti-Serum Response Factor/SRF Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Serum Response Factor/SRF Antibody Picoband® (ABIN7600213). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human SRF recombinant protein (Position: K163-E508).
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亚型
- IgG
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anti-Serum Response Factor (SRF) (AA 101-250) antibody
SRF 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (AA 406-508) antibodySRF 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E1 unconjugated
anti-Serum Response Factor (SRF) (pSer99) antibodySRF 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (AA 406-508) antibodySRF 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Serum Response Factor (SRF) (N-Term) antibodySRF 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (AA 57-269) antibodySRF 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (AA 616-875) antibodySRF 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (pSer77) antibodySRF 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (AA 231-474) antibodySRF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (N-Term) antibodySRF 适用: 人, 小鼠, 大鼠, Pig, 犬, Cow, 兔, 斑马鱼 WB, ChIP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Barron, M. R., Belaguli, N. S., Zhang, S. X., Trinh, M., Iyer, D., Merlo, X., Lough, J. W., Parmacek, M. S., Bruneau, B. G., Schwartz, R. J. Serum response factor, an enriched cardiac mesoderm obligatory factor, is a downstream gene target for Tbx genes. J. Biol. Chem. 280: 11816-11828, 2005. 2. Bell, R. D., Deane, R., Chow, N., Long, X., Sagare, A., Singh, I., Streb, J. W., Guo, H., Rubio, A., Van Nostrand, W., Miano, J. M., Zlokovic, B. V. SRF and myocardin regulate LRP-mediated amyloid-beta clearance in brain vascular cells. Nature Cell Biol. 11: 143-153, 2009. 3. Chang, J., Wei, L., Otani, T., Youker, K. A., Entman, M. L., Schwartz, R. J. Inhibitory cardiac transcription factor, SRF-N, is generated by caspase 3 cleavage in human heart failure and attenuated by ventricular unloading. Circulation 108: 407-413, 2003. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- SRF (Serum Response Factor (SRF))
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别名
- SRF
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背景
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Synonyms: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
Tissue Specificity: Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.
Background: Serum response factor, also known as SRF, is a transcription factor protein. This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene.
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分子量
- 67 kDa
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基因ID
- 6722
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UniProt
- P11831
抗原
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