EEF2K 抗体 (AA 162-719)
Quick Overview for EEF2K 抗体 (AA 162-719) (ABIN7600209)
抗原
See all EEF2K 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 162-719
-
原理
- Anti-EEF2K Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-EEF2K Antibody Picoband® (ABIN7600209). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human EEF2K recombinant protein (Position: K162-A719).
-
亚型
- IgG
-
-
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (Internal Region) antibody
EEF2K 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (N-Term) antibodyEEF2K 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 1-725) antibodyEEF2K 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 406-725) antibodyEEF2K 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 201-500) antibodyEEF2K 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (Tyr705) antibodyEEF2K 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 331-380), (pSer366) antibodyEEF2K 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (pSer366) antibodyEEF2K 适用: 人, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 337-371), (Ser366) antibodyEEF2K 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB56632 unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 337-371), (pSer366) antibodyEEF2K 适用: 人 WB 宿主: 兔 Polyclonal RB56631 unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Arora, S., Yang, J.-M., Craft, J., Hait, W. Detection of anti-elongation factor 2 kinase (calmodulin-dependent protein kinase III) antibodies in patients with systemic lupus erythematosus. Biochem. Biophys. Res. Commun. 293: 1073-1076, 2002. 2. Autry, A. E., Adachi, M., Nosyreva, E., E. S., Los, M. F., Cheng, P., Kavalali, E. T., Monteggia, L. M. NMDA receptor blockade at rest triggers rapid behavioural antidepressant responses. Nature 475: 91-95, 2011. 3. Ryazanov, A. G., Ward, M. D., Mendola, C. E., Pavur, K. S., Dorovkov, M. V., Wiedmann, M., Erdjument-Bromage, H., Tempst, P., Parmer, T. G., Prostko, C. R., Germino, F. J., Hait, W. N. Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase. Proc. Nat. Acad. Sci. 94: 4884-4889, 1997. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
-
-
- EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))
-
别名
- EEF2K
-
背景
-
Synonyms: Arachidonate 12-lipoxygenase, 12S-type,12S-LOX,12S-lipoxygenase,1.13.11.31,Lipoxin synthase 12-LO,3.3.2.-,Platelet-type lipoxygenase 12,ALOX12,12LO, LOG12,
Tissue Specificity: Expressed in vascular smooth muscle cells. .
Background: Eukaryotic elongation factor-2 kinase (eEF-2 kinase or eEF-2K), also known as calmodulin-dependent protein kinase III (CAMKIII) and calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, is an enzyme that in humans is encoded by the EEF2K gene. This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
-
分子量
- 82 kDa
-
基因ID
- 29904
-
UniProt
- O00418
-
途径
- AMPK Signaling
抗原
-
