EIF3E 抗体 (AA 160-249)
Quick Overview for EIF3E 抗体 (AA 160-249) (ABIN7600190)
抗原
See all EIF3E 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 160-249
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原理
- Anti-EIF3E Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-EIF3E Antibody Picoband® (ABIN7600190). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human EIF3E recombinant protein (Position: A160-M249).
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (Middle Region) antibody
EIF3E 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 犬, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (AA 346-445) antibodyEIF3E 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (AA 1-445) antibodyEIF3E 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (AA 36-85) antibodyEIF3E 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 犬, 豚鼠, 马, 兔, 猴, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (AA 248-276) antibodyEIF3E 适用: 人, 斑马鱼 WB 宿主: 兔 Polyclonal RB40515 unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (N-Term) antibodyEIF3E 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 犬, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (C-Term) antibodyVerified EIF3E 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (AA 360-409) antibodyEIF3E 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 犬, 豚鼠, 马, 兔, 猴, Pig, Bat, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (AA 1-445) antibodyEIF3E 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) antibodyEIF3E 适用: 人, 小鼠, 大鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Asano K, Merrick WC, Hershey JW (October 1997). "The translation initiation factor eIF3-p48 subunit is encoded by int-6, a site of frequent integration by the mouse mammary tumor virus genome". J. Biol. Chem. 272 (38): 23477-80. 2. Miyazaki, S., Imatani, A., Ballard, L., Marchetti, A., Buttitta, F., Albertsen, H., Nevanlinna, H. A., Gallahan, D., Callahan, R. The chromosome location of the human homolog of the mouse mammary tumor-associated gene INT6 and its status in human breast carcinomas. Genomics 46: 155-158, 1997. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))
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别名
- EIF3E
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背景
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Synonyms: Eukaryotic translation initiation factor 3 subunit E ,eIF3e ,Eukaryotic translation initiation factor 3 subunit 6 ,Viral integration site protein INT-6 homolog,eIF-3 p48 ,EIF3E ,EIF3S6 , INT6 ,
Tissue Specificity: Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus. .
Background: Eukaryotic translation initiation factor 3 subunit E is a protein that in humans is encoded by the EIF3E gene. The human homolog of EIF3E is located on chromosome region 8q22-q23. It is composed of 13 exons that span 45 kb of genomic DNA. EIF3E is the component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis ts localization/assembly. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). And the eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
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分子量
- 52 kDa
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基因ID
- 3646
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UniProt
- P60228
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途径
- Ribonucleoprotein Complex Subunit Organization, Hepatitis C
抗原
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