MT-ND5 抗体 (AA 154-570)
Quick Overview for MT-ND5 抗体 (AA 154-570) (ABIN7600145)
抗原
See all MT-ND5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 154-570
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原理
- Anti-MT-ND5 Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-MT-ND5 Antibody Picoband® (ABIN7600145). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human MT-ND5 recombinant protein (Position: L154-Q570).
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Anderson, S., Bankier, A. T., Barrell, B. G., de Bruijn, M. H. L., Coulson, A. R., Drouin, J., Eperon, I. C., Nierlich, D. P., Roe, B. A., Sanger, F., Schreier, P. H., Smith, A. J. H., Staden, R., Young, I. G. Sequence and organization of the human mitochondrial genome. Nature 290: 457-465, 1981. 2. Arizmendi, J. M., Skehel, J. M., Runswick, M. J., Fearnley, I. M., Walker, J. E. Complementary DNA sequences of two 14.5 kDa subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Complementation of the primary structure of the complex. FEBS Lett. 313: 80-84, 1992. 3. Attardi, G., Chomyn, A., Doolittle, R. F., Mariottini, P., Ragan, C. I. Seven unidentified reading frames of human mitochondrial DNA encode subunits of the respiratory chain NADH dehydrogenase. Cold Spring Harbor Symp. Quant. Biol. 51: 103-114, 1986. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- MT-ND5 (Mitochondrially Encoded NADH Dehydrogenase 5 (MT-ND5))
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别名
- MT-ND5
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背景
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Synonyms: Protein Wnt-10a, WNT10A
Tissue Specificity: In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults.
Background: MT-ND5 is a gene of the mitochondrial genome coding for the NADH-ubiquinone oxidoreductase chain 5 protein (ND5). A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7), while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 5 (ND5) is most likely a component of the hydrophobic protein fragment of Complex I. Mutations in the gene encoding for ND5 are implicated in Mitochondrial neurogastrointestinal encephalomyopathy, an autosomal recessive multisystem disorder that results in dramatically elevated levels of circulating thymidine and deoxyuridine.
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分子量
- 67 kDa
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基因ID
- 4540
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UniProt
- P03915
抗原
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