PHGDH 抗体 (AA 15-533)
Quick Overview for PHGDH 抗体 (AA 15-533) (ABIN7600112)
抗原
See all PHGDH 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 15-533
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原理
- Anti-PHGDH Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-PHGDH Antibody Picoband® (ABIN7600112). Tested in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PHGDH recombinant protein (Position: L15-F533). Human PHGDH shares 94.4% and 94.2% amino acid (aa) sequence identity with mouse and rat PHGDH, respectively.
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亚型
- IgG
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anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 1-533) antibody
PHGDH 适用: 人 WB, ELISA, IHC (p), IF, IP 宿主: 小鼠 Monoclonal 4A3-1D6 unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (N-Term) antibodyKO Validated PHGDH 适用: 人 WB, IHC (p), IF, IP, IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 264-533) antibodyPHGDH 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 140-168) antibodyPHGDH 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 2-251) antibodyPHGDH 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 249-277) antibodyPHGDH 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 1-533) antibodyPHGDH 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 1-533) antibodyPHGDH 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (N-Term) antibodyPHGDH 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 249-277) antibodyPHGDH 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB20883 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL
1. Acuna-Hidalgo, R., Schanze, D., Kariminejad, A., Nordgren, A., Kariminejad, M. H., Conner, P., Grigelioniene, G., Nilsson, D., Nordenskjold, M., Wedell, A., Freyer, C., Wredenberg, A., and 18 others. Neu-Laxova syndrome is a heterogeneous metabolic disorder caused by defects in enzymes of the L-serine biosynthesis pathway. Am. J. Hum. Genet. 95: 285-293, 2014. 2. Baek, J. Y., Jun, D. Y., Taub, D., Kim, Y. H. Assignment of human 3-phosphoglycerate dehydrogenase (PHGDH) to human chromosome band 1p12 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 89: 6-7, 2000. 3. Benke, P. J., Hidalgo, R. J., Braffman, B. H., Jans, J., van Gassen, K. L. I., Sunbul, R., El-Hattab, A. W. Infantile serine biosynthesis defect due to phosphoglycerate dehydrogenase deficiency: variability in phenotype and treatment response, novel mutations, and diagnostic challenges. J. Child Neurol. 32: 543-549, 2017. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- PHGDH (phosphoglycerate Dehydrogenase (PHGDH))
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别名
- PHGDH
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背景
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Synonyms: PHGDH, PGDH3, D-3-phosphoglycerate dehydrogenase, 3-PGDH, EC 1.1.1.95, 2-oxoglutarate reductase, EC 1.1.1.399, Malate dehydrogenase, EC 1.1.1.37
Background: This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
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分子量
- 57 kDa
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基因ID
- 26227
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UniProt
- O43175
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途径
- Metabolism of Steroid Hormones and Vitamin D, Warburg Effect
抗原
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