ASH2L 抗体 (AA 142-628)
Quick Overview for ASH2L 抗体 (AA 142-628) (ABIN7600053)
抗原
See all ASH2L 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 142-628
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原理
- Anti-ASH2L Antibody Picoband®
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产品特性
- Anti-ASH2L Antibody Picoband® (ABIN7600053). Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human ASH2L recombinant protein (Position: N142-P628). Human ASH2L shares 99.2% amino acid (aa) sequence identity with mouse ASH2L.
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anti-Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L) (AA 493-628) antibody
ASH2L 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 6F6B9 unconjugated
anti-Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L) (AA 1-534) antibodyASH2L 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L) (AA 288-337) antibodyASH2L 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 豚鼠, 猴, Bat, 兔, Pig, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L) antibodyASH2L 适用: 人 WB, ICC, FACS, DB 宿主: 小鼠 Monoclonal 2046D2a unconjugated
anti-Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L) (AA 1-534) antibodyASH2L 适用: 人, 小鼠 WB, ELISA, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L) (AA 329-628) antibodyASH2L 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L) antibodyASH2L 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L) (AA 347-382) antibodyASH2L 适用: 小鼠 WB 宿主: 兔 Polyclonal RB52789 unconjugated
anti-Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L) (AA 424-530) antibodyASH2L 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B4 unconjugated
anti-Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L) (AA 424-530) antibodyASH2L 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4G7 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 3/14/2014. 2. Ikegawa, S., Isomura, M., Koshizuka, Y., Nakamura, Y. Cloning and characterization of ASH2L and Ash2l, human and mouse homologs of the Drosophila ash2 gene. Cytogenet. Cell Genet. 84: 167-172, 1999. 3. Li, Y., Han, J., Zhang, Y., Cao, F., Liu, Z., Li, S., Wu, J., Hu, C., Wang, Y., Shuai, J., Chen, J., Cao, L., Li, D., Shi, P., Tian, C., Zhang, J., Dou, Y., Li, G., Chen, Y., Lei, M. Structural basis for activity regulation of MLL family methyltransferases. Nature 530: 447-452, 2016. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))
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别名
- ASH2L
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背景
- Set1/Ash2 histone methyltransferase complex subunit ASH2 is an enzyme that in humans is encoded by the ASH2L gene. The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator. While yeast contain only one known Set1 protein, six Set1-related proteins exist in mammals: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4. These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30. These subunits are required for proper complex assembly and modulation of histone methyltransferase activity. MLL1 and MLL2 complexes contain the additional protein subunit, menin. Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4. MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis.
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分子量
- 69,80 kDa
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基因ID
- 9070
抗原
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