UBE2N 抗体 (AA 13-152)
Quick Overview for UBE2N 抗体 (AA 13-152) (ABIN7599924)
抗原
See all UBE2N 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 13-152
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原理
- Anti-Ubc13/UBE2N Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Ubc13/UBE2N Antibody Picoband® (ABIN7599924). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Ubc13/UBE2N recombinant protein (Position: Q13-I152).
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亚型
- IgG
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anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) antibody
UBE2N 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3G1-D10 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) antibodyUBE2N 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (Middle Region) antibodyUBE2N 适用: 人, 大鼠, 小鼠, 斑马鱼, 犬, Cow, 绵羊, 兔, 马, 豚鼠, Saccharomyces cerevisiae, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (Middle Region) antibodyUBE2N 适用: 人, 大鼠, 小鼠, 斑马鱼, 犬, Cow, 绵羊, 兔, 马, 豚鼠, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 41-74) antibodyUBE2N 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB49505 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 56-152) antibodyUBE2N 适用: 大鼠, 小鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 20-138) antibodyUBE2N 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) antibodyUBE2N 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4C11-G11 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) antibodyUBE2N 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (C-Term) antibodyUBE2N 适用: 人, 大鼠, 小鼠, 斑马鱼, Cow, 绵羊, 兔, 马, 豚鼠, Drosophila melanogaster, 小鸡, 非洲爪蟾, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, J., Hao, L., Li, C., Ye, B., Du, Y., Zhang, H., Long, B., Zhu, P., Liu, B., Yang, L., Li, P., Tian, Y., Fan, Z. The endoplasmic reticulum adaptor protein ERAdP initiates NK cell activation via the Ubc13-mediated NF-kappa-B pathway. J. Immun. 194: 1292-303, 2015. 2. Fukushima, T., Matsuzawa, S., Kress, C. L., Bruey, J. M., Krajewska, M., Lefebvre, S., Zapata, J. M., Ronai, Z., Reed, J. C. Ubiquitin-conjugating enzyme Ubc13 is a critical component of TNF receptor-associated factor (TRAF)-mediated inflammatory responses. Proc. Nat. Acad. Sci. 104: 6371-6376, 2007. 3. Gross, M. B. Personal Communication. Baltimore, Md. 7/25/2014. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))
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别名
- UBE2N
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背景
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Synonyms: Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA
Tissue Specificity: Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
Background: Ubiquitin-conjugating enzyme E2 N is a protein that in humans is encoded by the UBE2N gene. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
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分子量
- 17 kDa
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基因ID
- 7334
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UniProt
- P61088
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway
抗原
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