TOP3B 抗体 (AA 128-824)
Quick Overview for TOP3B 抗体 (AA 128-824) (ABIN7599907)
抗原
See all TOP3B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 128-824
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原理
- Anti-TOP3B Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-TOP3B Antibody Picoband® (ABIN7599907). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human TOP3B recombinant protein (Position: E128-R824).
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亚型
- IgG
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anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-862) antibody
TOP3B 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 4F11 unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-862) antibodyTOP3B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1H4 unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-250) antibodyTOP3B 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (C-Term) antibodyTOP3B 适用: 人, 小鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (N-Term) antibodyTOP3B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) antibodyTOP3B 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-862) antibodyTOP3B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-270) antibodyTOP3B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kaufman, C. S., Genovese, A., Butler, M. G. Deletion of TOP3B is associated with cognitive impairment and facial dysmorphism. Cytogenet. Genome Res. 150: 106-111, 2016. 2. Kawasaki, K., Minoshima, S., Nakato, E., Shibuya, K., Shintani, A., Schmeits, J. L., Wang, J., Shimizu, N. One-megabase sequence analysis of the human immunoglobulin gamma gene locus. Genome Res. 7: 250-261, 1997. 3. Kwan, K. Y., Moens, P. B., Wang, J. C. Infertility and aneuploidy in mice lacking a type IA DNA topoisomerase III-beta. Proc. Nat. Acad. Sci. 100: 2526-2531, 2003. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- TOP3B (Topoisomerase (DNA) III beta (TOP3B))
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别名
- TOP3B
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背景
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Synonyms: E3 ubiquitin-protein ligase RNF169, RING finger protein 169, RING-type E3 ubiquitin transferase RNF169, RNF169, KIAA1991
Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.
Background: DNA topoisomerase 3-beta-1 is an enzyme that in humans is encoded by the TOP3B gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
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分子量
- 90-97 kDa
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基因ID
- 8940
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UniProt
- O95985
抗原
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