TOP3B 抗体 (C-Term)
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- 抗原 See all TOP3B 抗体
- TOP3B (Topoisomerase (DNA) III beta (TOP3B))
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抗原表位
- C-Term
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TOP3B antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- TOP3B Antibody detects endogenous levels of total TOP3B.
- 预测反应
- Rabbit
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human TOP3B, corresponding to a region within C-terminal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product TOP3B Primary Antibody
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anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-862) antibody
TOP3B 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 4F11 unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-862) antibodyTOP3B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1H4 unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-250) antibodyTOP3B 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (N-Term) antibodyTOP3B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) antibodyTOP3B 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-862) antibodyTOP3B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-270) antibodyTOP3B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- TOP3B (Topoisomerase (DNA) III beta (TOP3B))
- 别名
- TOP3B (TOP3B 产品)
- 别名
- TOP3B1 antibody, top3b antibody, DNA topoisomerase III beta antibody, topoisomerase (DNA) III beta antibody, DNA topoisomerase III beta L homeolog antibody, TOP3B antibody, Top3b antibody, top3b.L antibody
- 背景
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Description: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.
Gene: TOP3B
- 分子量
- 96 kDa
- 基因ID
- 8940
- UniProt
- O95985
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