SART1 抗体 (AA 125-800)
Quick Overview for SART1 抗体 (AA 125-800) (ABIN7599884)
抗原
See all SART1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 125-800
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原理
- Anti-SART1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-SART1 Antibody Picoband® (ABIN7599884). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human SART1 recombinant protein (Position: K125-K800).
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亚型
- IgG
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anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) (AA 1-255) antibody
SART1 适用: 人 WB, IP, IHC 宿主: 兔 Polyclonal unconjugated
anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) (Internal Region) antibodySART1 适用: 人, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) (AA 1-800) antibodySART1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) (C-Term) antibodySART1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) antibodySART1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) (N-Term) antibodyVerified SART1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) (C-Term) antibodySART1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) antibodySART1 适用: 人, 小鼠, 大鼠 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 3/1/2021. 2. Kittler, R., Putz, G., Pelletier, L., Poser, I., Heninger, A.-K., Drechsel, D., Fischer, S., Konstantinova, I., Habermann, B., Grabner, H., Yaspo, M.-L., Himmelbauer, H., Korn, B., Neugebauer, K., Pisabarro, M. T., Buchholz, F. An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature 432: 1036-1040, 2004. 3. Makarova, O. V., Makarov, E. M., Luhrmann, R. The 65 and 110 kDa SR-related proteins of the U4/U6-U5 tri-snRNP are essential for the assembly of mature spliceosomes. EMBO J. 20: 2553-2563, 2001. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- SART1 (Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1))
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别名
- SART1
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背景
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Synonyms: POU domain, class 4, transcription factor 1
Tissue Specificity: Expressed in the brain and the retina. Present in the developing brain, spinal cord and eye.
Background: U4/U6.U5 tri-snRNP-associated protein 1 is a protein that in humans is encoded by the SART1 gene. This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation, its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway.
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分子量
- 110 kDa
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基因ID
- 9092
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UniProt
- O43290
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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